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BIBTEX RIS

99m Tc‐HYNIC‐Bombesin (7‐14)NH 2 : Radiochemical Evaluation with Co‐ligands EDDA (EDDA = Ethylenediamine‐N,N′‐diacetic Acid), Tricine, and Nicotinic Acid

2005; Marcel Dekker; Volume: 35; Issue: 1 Linguagem: Inglês

10.1081/sim-200047545

ISSN

1553-3182

Autores

Bluma Linkowski Faintuch, Rodrigo Luis Silva Ribeiro Santos, A. L. F. M. Souza, Timothy J. Hoffman, Michael A. Greeley, Charles J. Smith,

Tópico(s)

Lung Cancer Treatments and Mutations

Resumo

Bombesin (BBN) is a peptide exhibiting high affinity for the gastrin releasing peptide receptor (GRPr), which is over‐expressed on a variety of human tumors including breast, prostate, lung and pancreatic cancers. The specific aim of this study was to identify a new 99mTc‐radiolabeled BBN analogue based upon the bifunctional chelating ligand HYNIC (2‐hydrazinonicotinamide) that might be used as a noninvasive tool for diagnosis of GRP receptor‐positive tumors. In this study, HYNIC‐β‐Ala‐BBN(7‐14)NH2 and HYNIC‐5‐Ava‐BBN(7‐14)NH2 were synthesized using traditional solid phase peptide synthetic techniques. The newly‐formed conjugates were radiolabeled using 99mTc in the presence of different coligands including tricine, ethylenediamine diacetic acid (EDDA), tricine/EDDA, and tricine/nicotinic acid. Radiolabeling conditions (i.e., pH, temperature, and reaction time) were optimized and evaluated by ITLC and reversed‐phase HPLC.

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