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Analytical Aspects of Plant Metabolite Profiling Platforms: Current Standings and Future Aims

2006; American Chemical Society; Volume: 6; Issue: 2 Linguagem: Inglês

10.1021/pr0604716

1535-3907

Christoph Seger, Sonja Sturm,

Plant biochemistry and biosynthesis

Over the past years, metabolic profiling has been established as a comprehensive systems biology tool. Mass spectrometry or NMR spectroscopy-based technology platforms combined with unsupervised or supervised multivariate statistical methodologies allow a deep insight into the complex metabolite patterns of plant-derived samples. Within this review, we provide a thorough introduction to the analytical hard- and software requirements of metabolic profiling platforms. Methodological limitations are addressed, and the metabolic profiling workflow is exemplified by summarizing recent applications ranging from model systems to more applied topics. Keywords: metabolic profiling • metabolomics • mass spectrometry • NMR • hyphenated techniques • multivariate statistical methods • plant metabolism • primary metabolites • secondary metabolites • phytopharmaceuticals • genetically modified organisms