Portal de Periódicos da CAPES

Artigo Produção Nacional Revisado por pares

BIBTEX RIS

Local delivery of EGF–liposome mediated bone modeling in orthodontic tooth movement by increasing RANKL expression

2009; Elsevier BV; Volume: 85; Issue: 19-20 Linguagem: Inglês

10.1016/j.lfs.2009.09.010

1879-0631

José Bento Alves, Cynthia Lopes Ferreira, Aline Fagundes Martins, Gerluza A.B. Silva, Guilherme Dias Alves, Tony P. Paulino, Pietro Ciancaglini, Geraldo Thedei, Marcelo Henrique Napimoga,

Bone health and osteoporosis research

It has long been demonstrated that epidermal growth factor (EGF) has catabolic effects on bone. Thus, we examined the role of EGF in regulating mechanically induced bone modeling in a rat model of orthodontic tooth movement.The maxillary first molars of rats were moved mesially using an orthodontic appliance attached to the maxillary incisor teeth. Rats were randomly divided into 4 groups: (G1) administration of PBS (phosphate buffer saline) solution (n=24); (G2) administration of empty liposomes (n=24); (G3) administration 20ng of EGF solution (n=24); and (G4) 20ng of EGF-liposomes solution (n=24). Each solution was injected in the mucosa of the left first molar adjacent to the appliance. At days 5, 10, 14 and 21 after drug administration, 6 animals of each group were sacrificed. Histomorphometric analysis was used to quantify osteoclasts (Tartrate-resistant acid phosphatase (TRAP)+cells) and tooth movement. Using immunohistochemistry assay we evaluated the RANKL (receptor activator of nuclear factor kappaB ligand) and epidermal growth factor receptor (EGFR) expression.The EGF-liposome administration showed an increased tooth movement and osteoclast numbers compared to controls (p<0.05). This was correlated with intense RANKL expression. Both osteoblasts and osteoclasts expressed EGFR.Local delivery of EGF-liposome stimulates osteoclastogenesis and tooth movement.