Retrograde labelling of dorsal root ganglion cells in the rat: A quantitative and morphological comparison of Fluoro-Gold with horseradish peroxidase labelling
1992; Elsevier BV; Volume: 141; Issue: 1 Linguagem: Inglês
10.1016/0304-3940(92)90332-2
ISSN1872-7972
AutoresA. Baranowski, Uma Anand, Stephen B. McMahon,
Tópico(s)Immunotoxicology and immune responses
ResumoWe have compared retrograde labelling of rat primary sensory neurons using Fluoro-Gold (FG) and horseradish peroxide conjugated with wheat germ agglutinin (HRP-WGA). Fluoro-Gold 2.5% after 48 h transit time and FG 5% after 24 and 48 h retrogradely labelled similar numbers of cell profiles as HRP-WGA (P greater than 0.1% Student's t-test). The calculated cell size distribution for the above FG groups were similar to those for the HRP-WGA. However, FG 2.5% after a 24 h transit time labelled significantly fewer cells (P less than 0.001 Student's t-test). FG retrograde transport may be used to identify the same population of DRG cells as HRP-WGA.
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