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Drug-target interactions on a single living cell: an approach by optical microspectroscopy

1991; SPIE; Volume: 1403; Linguagem: Inglês

10.1117/12.57316

1996-756X

Michel Manfait, Hamid Morjani, Jean‐Marc Millot, Vincent Debal, Jean‐François Angiboust, Igor Nabiev,

Spectroscopy and Chemometric Analyses

Optical microspectroscopic techniques - both powerful and nondestructive - are particularly suited to the study within a single living cell of molecular interactions between drugs (anticancer agents or xenobiotics) and their pharmacological targets. These techniques include vibrational spectroscopy : Raman spectroscopy (conventional resonance SERS) and Fourier transform infrared microspectroscopy together with fluorescence microspectroscopy. Applications are given Ci) in the subcompartmental quantitative analysis of anthracyclines and ymca-alkaloids and (ii) in the study within the cell of the molecular interactions of these compounds with their target molecules. Among these techniques laser microspectrofluorometry is easy to operate and allows the study of intracellular drug pharmacokinetics related to biological characteristics such as cell resistance or differentiation.