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CNS neuronal focal adhesion kinase forms clusters that co-localize with vinculin

1996; Wiley; Volume: 46; Issue: 4 Linguagem: Inglês

10.1002/(sici)1097-4547(19961115)46

1097-4547

Gerin R. Stevens, Chi Zhang, Margaret M. Berg, Mary P. Lambert, Kirsten Barber, Isabel Cantallops, Aryeh Routtenberg, William L. Klein,

Hippo pathway signaling and YAP/TAZ

Journal of Neuroscience ResearchVolume 46, Issue 4 p. 445-455 Full Access CNS neuronal focal adhesion kinase forms clusters that co-localize with vinculin Gerin R. Stevens, Gerin R. Stevens Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorChi Zhang, Chi Zhang Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorMargaret M. Berg, Margaret M. Berg Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorMary P. Lambert, Mary P. Lambert Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorKirsten Barber, Kirsten Barber Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorIsabel Cantallops, Isabel Cantallops Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorAryeh Routtenberg, Aryeh Routtenberg Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorWilliam L. Klein, Corresponding Author William L. Klein Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Department of Neurobiology and Physiology, 2153 North Campus Drive, Northwestern University, Evanston, IL 60208Search for more papers by this author Gerin R. Stevens, Gerin R. Stevens Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorChi Zhang, Chi Zhang Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorMargaret M. Berg, Margaret M. Berg Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorMary P. Lambert, Mary P. Lambert Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorKirsten Barber, Kirsten Barber Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorIsabel Cantallops, Isabel Cantallops Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorAryeh Routtenberg, Aryeh Routtenberg Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Search for more papers by this authorWilliam L. Klein, Corresponding Author William L. Klein Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208Department of Neurobiology and Physiology, 2153 North Campus Drive, Northwestern University, Evanston, IL 60208Search for more papers by this author First published: 15 November 1996 https://doi.org/10.1002/(SICI)1097-4547(19961115)46:4 3.0.CO;2-GCitations: 24AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that appears to play a central role in integrin-mediated signal transduction in non-neuronal cells, linking the extracellular matrix to the actin-based cytoskeleton at focal adhesion contacts. Biochemical analysis has revealed the presence of FAK immunoreactivity in cells of neuronal lineage (Zhang et al., 1994) and in the CNS (Burgaya et al. 1995; Grant et al., 1995). In the current work, we have examined the immunodistribution of FAK in nerve cell cultures and tissue sections from the rat CNS. Cultures of B103 CNS neuroblastoma cells and primary cultures of hippocampal neurons both showed abundant FAK immunoreactivity in nerve cell bodies. Immunoreactivity also extended into neurites and growth cones. The most striking feature of FAK distribution was the presence of short, punctate clusters of high FAK concentration. These FAK clusters were maintained in triton-extracted cell ghosts, indicating association with the cytoskeleton. Double-label confocal imaging showed that clusters of FAK coincided with clusters of vinculin, another actin-associated signal transduction molecule implicated in control of growth cone motility. Data from hippocampal sections verified the presence of FAK in adult neurons where it was enriched in somato-dendritic domains and showed a non-uniform distribution. Quantitative FAK immunoprecipitation to compare adult with embryonic brain showed a 7-fold developmental down-regulation of FAK and a 21-fold down-regulation of FAK TyrP. The data suggest that neuronal FAK may participate in signal transduction complexes relevant to neuronal morphogenesis and plasticity. © 1996 Wiley-Liss, Inc. References Abe K, Saito H (1993): Tyrosine kinase inhibitors, herbimycin A and lavendustin A, block formation of long-term, potentiation in the dentate gyrus in vivo. Brain Res 621: 167–170. 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