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High-resolution1H and1H-13C magic angle spinning NMR spectroscopy of rat liver

2000; Wiley; Volume: 44; Issue: 2 Linguagem: Inglês

10.1002/1522-2594(200008)44

1522-2594

Mary E. Bollard, S. Garrod, Elaine Holmes, John C. Lindon, Eberhard Humpfer, Manfred Spraul, Jeremy K. Nicholson,

NMR spectroscopy and applications

High-resolution magic angle spinning (MAS) (1)H NMR spectra of small samples (ca. 8 mg) of intact rat liver are reported for the first time. One dimensional spectra reveal a number of large well-resolved NMR signals mainly from low to medium molecular weight compounds (generally 4) linked glucose units with either alpha(1-->4) units adjacent or alpha(1-->6) linked branches could be resolved in a high-resolution (1)H NMR experiment giving direct in situ information on the ratio of alpha(1-->4) to alpha(1-->6) units. This indicates that despite the relatively high MW (>1,000,000 Daltons) there is considerable segmental motion in the glycogen molecules giving long (1)H T(2) relaxation times. Magn Reson Med 44:201-207, 2000.