Ethoxyresorufin-O-deethylase (EROD) activity in flatfish: Fast determination with a fluorescence plate-reader
1992; Elsevier BV; Volume: 33; Issue: 3 Linguagem: Inglês
10.1016/0141-1136(92)90149-g
ISSN1879-0291
AutoresMartin L. Eggens, François Galgani,
Tópico(s)Analytical Chemistry and Chromatography
ResumoEthoxyresorufin-O-deethylase (EROD) is one of the model reactions of the cytochrome P-4501A1 mediated monooxygenase (MO) system. It is often used to detect the effects of increased concentrations of planar aromatic microcontaminants. The comparison of a new and faster method for measuring the EROD-activity using a plate-reader with the conventional method showed an almost equal sensitivity and precision between the two methods. Characteristic in the use of the plate-reader method is that the protein concentration of the (diluted) sample has to be in the range of 0·9–3 mg ml−1. In this range the EROD-kinetics are linear for at least 8 min. The compactness and rigidity of the equipment and the speed of analysis makes the plate-reader method very suitable for research onboard of a research vessel.
Referência(s)