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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography

2007; Wiley; Volume: 30; Issue: 16 Linguagem: Inglês

10.1002/jssc.200700228

ISSN

1615-9314

Autores

João Paulo Barreto Sousa, Paula Carolina Pires Bueno, Luiz Elídio Gregório, Ademar Alves da Silva Filho, Niege Araçari Jacometti Cardoso Furtado, Márcia L. de Sousa, Jairo Kenupp Bastos,

Tópico(s)

Insect and Pesticide Research

Resumo

A sensitive and reliable RP-HPLC method was developed using a C18 CLC-ODS (M) - 4.6x250 mm(2)column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83-533.33 microg/mL.

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