Effect of Chelating Agents on Plasmodium Formation by the True Slime Mould, Didymium nigripes
1960; Nature Portfolio; Volume: 188; Issue: 4757 Linguagem: Inglês
10.1038/1881206a0
ISSN1476-4687
Autores Tópico(s)Biocrusts and Microbial Ecology
ResumoSPORES of Didymium nigripes, a true slime mould, germinate to liberate myxamœbæ which multiply by binary fission. After a period of exponential growth, the uninucleate myxamœbæ fuse to form macroscopic, multinucleate veins of rhythmically flowing protoplasm called plasmodia. Following an indefinite period of growth, the plasmodia differentiate into spore-containing fruiting bodies. Kerr and Sussman1 grew D. nigripes in monoxenic culture with Aerobacter aerogenes on a dilute glucose–peptone–yeast extract agar medium buffered with phosphate at pH 6.2 (hereafter called GPY/5). All stages of the life-cycle were regularly reproduced in less than 7 days. When 0.25 per cent (w/v) brucine was added to the GPY/5, plasmodia did not appear. Instead, the myxamœbæ remained indefinitely as such and could be sub-cultured as amœbæ The myxamœbæ quickly formed plasmodia when harvested from the GPY/5-brucine plates and washed by differential centrifugation.
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