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Development of Rapid Somatic Cell Counting Method by Using Dye Adding NIR Spectroscopy

2008; Korean Society for Food Science of Animal Resources; Volume: 28; Issue: 1 Linguagem: Inglês

10.5851/kosfa.2008.28.1.63

2234-246X

Ke-Sung Kim, Hae-Won Noh, Sang-Dong Lim, Changhyun Choi, Yong–Joo Kim,

Spectroscopy Techniques in Biomedical and Chemical Research

본 연구에서는 근적외선 분광광도계를 이용하여 원유의 체세포수 측정에 필요한 최적의 색소를 선정하기 위하여 eosin-Y, methyl red, methylen blue, resazurin 및 amido black 10B등의 5가지 색소를 0.01%첨가 후 근적외선 분광광도계를 이용하여 400-2,500 nm 영역에서 측청하였으며, PLS(partial least squar)분석 결과는 다음과 같다. 교정부 모델에서의 상관도는 raw milk 0.78, eosin-Y 0.65, methyl red 0.63, methylen blue 0.65, resazurin 0.98 그리고 amido black 10B는 0.99 였다. 또한, 검증부 모델에서의 상관도는 raw milk 0.49, eosin-Y 0.21, methyl red 0.36, methylen blue 0.47, resazurin 0.95 그리고 amido black 10B는 0.98 였다. 위 결과를 종합해 보면, amido black 10B를 첨가한 경우 검증부 모델의 상관도는 0.98, 검증부 오차(SEP)는 0.09로 가장 우수한 결과를 보였다. To develop the somatic cell counting NIR Spectrum method within a range of 400-2500 nm, eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were tested at 0.01% in raw milk. The PLS (Partial Least Square) results are summarized as follows: Correlation coefficients of the calibration model measurements by NIR spectroscopy in raw milk for eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were 0.78, 0.65, 0.63, 0.65, 0.98 and 0.99, respectively. The correlation coefficients of the prediction model measurements by NIR spectroscopy in raw milk for eosin-Y, methyl red, methylene blue, resazurin and amido black 10B were 0.49, 0.21, 0.36, 0.47, 0.95 and 0.98 respectively. Based on these results, amido black 10B was the best additive for the NIRS somatic cell count method.