Studies in Red Blood Cell Preservation: 5. Determining the Limiting Concentrations of NH 4 Cl and Na 2 HPO 4 Needed to Maintain Red Blood Cell ATP during Storage
1992; Wiley; Volume: 62; Issue: 3 Linguagem: Inglês
10.1111/j.1423-0410.1992.tb01186.x
ISSN1423-0410
AutoresU.J. Dumaswala, R. T. Oreskovic, T.L. Petrosky, T. J. Greenwalt,
Tópico(s)Hemoglobinopathies and Related Disorders
ResumoAbstract The purpose of the present study was to define the lowest concentrations of ammonium (NH 4 +) and phosphate (Pi) in an experimental additive solution (EAS) that would support suitable red blood cell (RBC) ATP levels and other in vitro characteristics for at least 84 days. It was determined that ATP maintenance was dependent upon both NH 4 + and Pi concentrations. RBCs stored for 84 days in additive solutions containing 10 m M NH 4 + and 0,15, 25 and 40 m M Pi had ATP values averaging 1.87, 2.49, 2.70 and 2.65 μmol/g Hb, respectively. The shedding of exocytic hemoglobin‐containing vesicles and percent hemolysis were significantly (p < 0.001) elevated in the preservative containing 40 m M Pi. These data suggest that an EAS containing 10 m M NH 4 + and 15 m M Pi would be optimal for storing RBCs up to 84 days. The extended storage would be particularly advantageous for autologous transfusion programs.
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