Artigo Acesso aberto Produção Nacional Revisado por pares

Development and evaluation of an edible antimicrobial film based on yam starch and chitosan

2005; Wiley; Volume: 19; Issue: 1 Linguagem: Inglês

10.1002/pts.713

ISSN

1099-1522

Autores

Alba Durango, Nilda de Fátima Ferreira Soares, S. D. Benevides, J. A. Teixeira, Márcia Maria de Carvalho, Carmen Wobeto, N. J. Andrade,

Tópico(s)

Medicinal Plants and Neuroprotection

Resumo

Packaging Technology and ScienceVolume 19, Issue 1 p. 55-59 Research Article Development and evaluation of an edible antimicrobial film based on yam starch and chitosan A. M. Durango, A. M. Durango Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorN. F. F. Soares, Corresponding Author N. F. F. Soares [email protected] Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilUniversidade Federal de Vicosa, Departemento de Techologia de Alimentos, 36570-000, Vicosa – MG, BrazilSearch for more papers by this authorS. Benevides, S. Benevides Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorJ. Teixeira, J. Teixeira Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorM. Carvalho, M. Carvalho Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorC. Wobeto, C. Wobeto Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorN. J. Andrade, N. J. Andrade Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this author A. M. Durango, A. M. Durango Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorN. F. F. Soares, Corresponding Author N. F. F. Soares [email protected] Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilUniversidade Federal de Vicosa, Departemento de Techologia de Alimentos, 36570-000, Vicosa – MG, BrazilSearch for more papers by this authorS. Benevides, S. Benevides Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorJ. Teixeira, J. Teixeira Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorM. Carvalho, M. Carvalho Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorC. Wobeto, C. Wobeto Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this authorN. J. Andrade, N. J. Andrade Universidade Federal de Viçosa, Departamento de Tecnologia de Alimentos 36570-000, Viçosa-MG, BrazilSearch for more papers by this author First published: 01 December 2005 https://doi.org/10.1002/pts.713Citations: 75AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Edible antimicrobial films are an innovation within the biodegradable active packaging concept. They have been developed in order to reduce and/or inhibit the growth of microorganisms on the surface of foods. This study developed an edible antimicrobial film based on yam starch (Dioscorea alata) and chitosan and investigated its antimicrobial efficiency on Salmonella enteritidis. A solution of yam starch (4%) and glycerol (2%) was gelatinized in a viscoamilograph and chitosan added at concentrations of 1%, 3% and 5%. Films with and without chitosan were produced by the cast method. To evaluate the antimicrobial activity of the films, two suspensions of S. enteritidis were used in BHI medium, corresponding to counts of 2 × 108 and 1.1 × 106 CFU/ml. The suspensions (50 ml) were poured into flasks. The films were cut into 5 × 5 and 5 × 10 cm rectangles to be used at ratios of 1 : 1 (1 cm2/ml microorganism suspension) and 2 : 1 (2 cm2/ml). The film 30 µm thick on average. As a control, pure chitosan at an amount corresponding to that contained in the 3% and 5% films (5 × 10 cm) was added to flasks containing the microorganism suspension. Also, flasks containing only a suspension of S. enteritidis were used as control. The suspensions, in flasks, were kept at 37°C in a waterbath with agitation. Suspension aliquots were removed every hour for reading the optic density (OD595) and plating onto PCA medium. The results showed that chitosan has a bactericidal effect upon S. enteritidis. Films treated with chitosan at different concentrations showed similar antimicrobial efficiency, in addition to being dependent on diffusion. The chitosan-treated films caused a reduction of one to two log cycles in the number of microorganisms, whereas the pure chitosan presented a reduction of four to six log cycles compared with the control and starch film. The films showed good flexibility. Copyright © 2005 John Wiley & Sons, Ltd. REFERENCES 1. Quintavalla S, Vicini L. Antimicrobial food packaging in meat industry. Meat Sci. 2002; 62: 373–380. 2. Appendini P, Hotchkiss JH. Review of antimicrobial food packaging. Innov. Food Sci. Emerg. Technol. 2002; 3: 113–126. 3. Padgett T, Han LY, Dawson PL. Incorporation of food-grade antimicrobial compounds into biodegradable packaging films. J. Food Protect. 1998; 61(10): 1330–1335. 4. Ouattara B, Simard R, Piette G, Bégin A, Holley RA. Inhibition of surface spoilage bacteria in processed meats by application of antimicrobial films prepared with chitosan. Int. J. Food Microbiol. 2000; 62: 139–148. 5. Vartiainen J, Skytta E, Ahvenainen Rantala R, Enqvist J. Antimicrobial and barrier properties of LDPE films containing imazalil and EDTA. J. Plastic Film Sheeting 2003; 19: 249–261. 6. Guilbert S, Gontard N, Gorris GM. Prolongation of the self-life of perishable food products using biodegradable films and coatings. Lebensm. Wissensch. Technol. 1996; 29(1–2): 10–17. 7. Gennadios A, Hanna MA, Kurth LB. Application of edible coatings on meats, poultry and seafoods: a review. Lebensm. Wissensch. Technol. 1997; 30(4): 337–350. 8. Lazaridou A, Biliaderis CG. Thermophysical properties of chitosan–starch and chitosan–pullulan films near the glass transition. Carbohydr. Polym. 2002; 48: 179–190. 9. Wang GH. Inhibition and inactivation of five species of foodborne pathogens by chitosan. J. Food Protect. 1992; 55(11): 916–919. 10. Darmadji P, Izumimoto M. Effect of chitosan in meat preservation. Meat Sci. 1994; 38: 243–254. 11. Jongrittiporn S, Kungsuwan A, Rakshit SK. Paper presented at the European Conference on Advanced Technology for Safe and High Quality Foods (EUROCAFT), Berlin, 2001. 12. Shahidi F, Arachchi JKV, Jeon YJ. Food application of chitin and chitosans. Trends Food Sci. Technol. 1999; 10: 37–51. 13. Romanazzi G, Nigro F, Hipólito A, Di Venere D, Salerno M. Effects of pre- and postharvest chitosan treatments to control storage grey mold of table grapes. J. Food Sci. 2002; 67(5): 1862–1866. 14. Soares NFF. Bitterness reduction in grapefruit juice through active packaging. Ph.D. Dissertation, Cornell University, 1997. 15. Coma V, Martial Giros A, Garreau S et al. Edible antimicrobial films based on chitosan matrix. J. Food Sci. 2002; 67(3): 1162–1168. 16. Cagri A, Ustunol Z, Ryser ET. Antimicrobial, mechanical, and moisture barrier properties of low pH whey protein-based edible films containing p-amminobenzoic or sorbic acid. J. Food Sci. 2001; 66(6): 865–870. 17. Brody AL, Strupinsky ER, Kline LR. Active Packaging for Food Applications. Technomic: Lancaster, 2001; 218. Citing Literature Volume19, Issue1January/February 2006Pages 55-59 ReferencesRelatedInformation

Referência(s)
Altmetric
PlumX