Improvement of antibody surface density by orientation of reduced fragments
1994; Elsevier BV; Volume: 247; Issue: 2 Linguagem: Inglês
10.1016/0040-6090(94)90807-9
ISSN1879-2731
AutoresArti Ahluwalia, Maria Gabriela Jacheto Carra, Danilo De Rossi, Chiara Ristori, Pietro Tundo, Andrea Bomben,
Tópico(s)Advanced biosensing and bioanalysis techniques
ResumoA procedure for the orientation of antibody fragments at the gas-water interface is described and preliminary results are presented. The method exploits the presence of the reactive sulfhydryl group of a reduced antibody fragment which can react with a floating monolayer. Surface pressure measurements show a strong interaction between the reduced fragments and the monolayer. Deposited films are characterised by a fluorescent immunoassay and ellipsometry and the results indicate that the antibody fragments are oriented with a high packaging densitiy.
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