An application of high-speed counter-current chromatography coupled with electrospray ionization mass spectrometry for separation and online identification of coumarins from Peucedanum praeruptorum Dunn
2009; Elsevier BV; Volume: 877; Issue: 24 Linguagem: Inglês
10.1016/j.jchromb.2009.06.042
ISSN1873-376X
AutoresZhiguo Hou, Deran Xu, Shun Yao, Jianguang Luo, Ling‐Yi Kong,
Tópico(s)Medicinal Plant Pharmacodynamics Research
ResumoA new and systematic application for separation and online identification of coumarins from Peucedanum praeruptorum Dunn by preparative high-speed counter-current chromatography coupled with electrospray ionization multi-stage mass spectrometry (prep-HSCCC/ESI-MS(n)) was established. The procedure of separation was guided by the chromatogram of ion current. The structures of acquisitions were deduced by MS information. The hyphenation between prep-HSCCC/ESI-MS(n) was designed to keep the split ratio from 1:20 to 1:200 exactly. Seven compounds were obtained and two new compounds were detected. It was proved that prep-HSCCC/ESI-MS(n) was an effective method for sensitive detection, rapid identification and separation of natural products.
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