Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution
2014; American Association for the Advancement of Science; Volume: 346; Issue: 6208 Linguagem: Inglês
10.1126/science.1257998
ISSN1095-9203
AutoresBi‐Chang Chen, Wesley R. Legant, Kai Wang, Lin Shao, Daniel E. Milkie, Michael W. Davidson, Chris Janetopoulos, Xufeng Wu, John A. Hammer, Zhe Liu, Brian P. English, Yuko Mimori‐Kiyosue, Daniel P. Romero, Alex T. Ritter, Jennifer Lippincott‐Schwartz, Lillian K. Fritz‐Laylin, R. Dyche Mullins, Diana M. Mitchell, Joshua N. Bembenek, Anne-Cécile Reymann, Ralph Böhme, Stephan W. Grill, Jennifer T. Wang, Géraldine Seydoux, U. Serdar Tulu, Daniel P. Kiehart, Eric Betzig,
Tópico(s)Near-Field Optical Microscopy
ResumoFrom single molecules to embryos in living color Animation defines life, and the three-dimensional (3D) imaging of dynamic biological processes occurring within living specimens is essential to understand life. However, in vivo imaging, especially in 3D, involves inevitable tradeoffs of resolution, speed, and phototoxicity. Chen et al. describe a microscope that can address these concerns. They used a class of nondiffracting beams, known as 2D optical lattices, which spread the excitation energy across the entire field of view while simultaneously eliminating out-of-focus excitation. Lattice light sheets increase the speed of image acquisition and reduce phototoxicity, which expands the range of biological problems that can be investigated. The authors illustrate the power of their approach using 20 distinct biological systems ranging from single-molecule binding kinetics to cell migration and division, immunology, and embryonic development. Science , this issue 10.1126/science.1257998
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