Portal de Periódicos da CAPES

Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line

1994; Royal Society; Volume: 258; Issue: 1353 Linguagem: Inglês

10.1098/rspb.1994.0178

1471-2954

Neil S. Millar, Steven D. Buckingham, David B. Sattelle,

Insect-Plant Interactions and Control

Restricted accessMoreSectionsView PDF ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmail Cite this article Millar Neil S. , Buckingham Steven D. and Sattelle David B. 1994Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell lineProc. R. Soc. Lond. B.258307–314http://doi.org/10.1098/rspb.1994.0178SectionRestricted accessArticleStable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line Neil S. Millar Google Scholar Find this author on PubMed Search for more papers by this author , Steven D. Buckingham Google Scholar Find this author on PubMed Search for more papers by this author and David B. Sattelle Google Scholar Find this author on PubMed Search for more papers by this author Neil S. Millar Google Scholar Find this author on PubMed , Steven D. Buckingham Google Scholar Find this author on PubMed and David B. Sattelle Google Scholar Find this author on PubMed Published:22 December 1994https://doi.org/10.1098/rspb.1994.0178AbstractA cloned Drosophila γ-aminobutyric acid (GABA) receptor subunit (Rdl) has been stably expressed as a functional homo-oligomeric ion channel in a Drosophila cell line. Stably-transfected clonal cell lines which expressed high levels of GABA receptor were identified by specific [3H]-muscimol binding. Expression of functional GABA-gated ion channels in these cell lines was demonstrated by electrophysiological recording. Rapid and pronounced rundown of responses to GABA during whole-cell patch clamp recordings was overcome by the inclusion of EGTA in the pipette solution, indicating a possible role for calcium-dependent processes in the functional regulation of this GABA receptor. Relative agonist potencies of the expressed receptor were found to be in the order GABA = TACA > CACA. We have observed a reversible block of the receptor by the convulsant antagonists, picrotoxinin and EBOB, and by the insecticide fipronil. Potentiation of GABA responses was seen with the anaesthetic steroid 5α-pregnan-3α-ol-20-one. No significant effects (either agonist, antagonist or modulatory) were observed with bicuculline (a vertebrate GABAAR antagonist), benzodiazepines or barbiturates (vertebrate GABAAR modulators), or with glycine (agonist of the closely related vertebrate glycine receptors). The suitability of this Drosophila stable expression system for the characterization of receptors and ion channels is discussed.FootnotesThis text was harvested from a scanned image of the original document using optical character recognition (OCR) software. As such, it may contain errors. Please contact the Royal Society if you find an error you would like to see corrected. Mathematical notations produced through Infty OCR. Previous ArticleNext Article VIEW FULL TEXT DOWNLOAD PDF FiguresRelatedReferencesDetailsCited by Liu G, Wu Y, Gao Y, Ju X and Ozoe Y (2020) Potential of Competitive Antagonists of Insect Ionotropic γ-Aminobutyric Acid Receptors as Insecticides, Journal of Agricultural and Food Chemistry, 10.1021/acs.jafc.9b08189, 68:17, (4760-4768), Online publication date: 29-Apr-2020. Price K and Lummis S (2014) An atypical residue in the pore of Varroa destructor GABA-activated RDL receptors affects picrotoxin block and thymol modulation, Insect Biochemistry and Molecular Biology, 10.1016/j.ibmb.2014.10.002, 55, (19-25), Online publication date: 1-Dec-2014. Liu G, Furuta K, Nakajima H, Ozoe F and Ozoe Y (2014) Competitive antagonism of insect GABA receptors by 4-substituted 5-(4-piperidyl)-3-isothiazolols, Bioorganic & Medicinal Chemistry, 10.1016/j.bmc.2014.07.021, 22:17, (4637-4645), Online publication date: 1-Sep-2014. Ribeiro S, D'Ambrosio M, Vale R and Drubin D (2014) Induction of focal adhesions and motility in Drosophila S2 cells , Molecular Biology of the Cell, 10.1091/mbc.e14-04-0863, 25:24, (3861-3869), Online publication date: 1-Dec-2014. Toret C, D'Ambrosio M, Vale R, Simon M and Nelson W (2014) A genome-wide screen identifies conserved protein hubs required for cadherin-mediated cell–cell adhesion, Journal of Cell Biology, 10.1083/jcb.201306082, 204:2, (265-279), Online publication date: 20-Jan-2014. Ozoe Y (2013) γ-Aminobutyrate- and Glutamate-gated Chloride Channels as Targets of Insecticides Target Receptors in the Control of Insect Pests: Part I, 10.1016/B978-0-12-394389-7.00004-1, (211-286), . Dong C, Hu A, Ni Y, Zuo Y and Li G (2013) Effects of midazolam, pentobarbital and ketamine on the mRNA expression of ion channels in a model organism Daphnia pulex, BMC Anesthesiology, 10.1186/1471-2253-13-32, 13:1, Online publication date: 1-Dec-2013. Ashby J, McGonigle I, Price K, Cohen N, Comitani F, Dougherty D, Molteni C and Lummis S (2012) GABA Binding to an Insect GABA Receptor: A Molecular Dynamics and Mutagenesis Study, Biophysical Journal, 10.1016/j.bpj.2012.10.016, 103:10, (2071-2081), Online publication date: 1-Nov-2012. Thompson A, McGonigle I, Duke R, Johnston G and Lummis S (2012) A single amino acid determines the toxicity of Ginkgo biloba extracts , The FASEB Journal, 10.1096/fj.11-192765, 26:5, (1884-1891), Online publication date: 1-May-2012. Nakao T, Kawase A, Kinoshita A, Abe R, Hama M, Kawahara N and Hirase K The A2′N Mutation of the RDL γ-Aminobutyric Acid Receptor Conferring Fipronil Resistance in Laodelphax striatellus (Hemiptera: Delphacidae), Journal of Economic Entomology, 10.1603/EC10391, 104:2, (646-652) Narahashi T (2010) Neurophysiological Effects of Insecticides Hayes' Handbook of Pesticide Toxicology, 10.1016/B978-0-12-374367-1.00031-8, (799-817), . Millar N and Lansdell S (2010) Characterisation of Insect Nicotinic Acetylcholine Receptors by Heterologous Expression Insect Nicotinic Acetylcholine Receptors, 10.1007/978-1-4419-6445-8_6, (65-73), . McGonigle I and Lummis S (2010) Molecular Characterization of Agonists That Bind to an Insect GABA Receptor, Biochemistry, 10.1021/bi901698c, 49:13, (2897-2902), Online publication date: 6-Apr-2010. Knipple D and Soderlund D (2010) The ligand-gated chloride channel gene family of Drosophila melanogaster, Pesticide Biochemistry and Physiology, 10.1016/j.pestbp.2009.09.002, 97:2, (140-148), Online publication date: 1-Jun-2010. Janssen D, Derst C, Rigo J and Van Kerkhove E (2010) Cys-Loop Ligand-Gated Chloride Channels in Dorsal Unpaired Median Neurons of Locusta migratoria , Journal of Neurophysiology, 10.1152/jn.00466.2009, 103:5, (2587-2598), Online publication date: 1-May-2010. Ozoe Y, Takeda M and Matsuda K (2009) γ-Aminobutyric Acid Receptors: A Rationale for Developing Selective Insect Pest Control Chemicals Biorational Control of Arthropod Pests, 10.1007/978-90-481-2316-2_6, (131-162), . Grünewald B and Wersing A (2008) An ionotropic GABA receptor in cultured mushroom body Kenyon cells of the honeybee and its modulation by intracellular calcium, Journal of Comparative Physiology A, 10.1007/s00359-007-0308-9, 194:4, (329-340), Online publication date: 1-Apr-2008. Basile G, Peticca M and Catello S (2007) Site-specific biotinylation of human myeloid differentiation protein 88 in Drosophila melanogaster Schneider 2 cell cytoplasm, Molecular Biotechnology, 10.1007/BF02686011, 35:3, (253-260), Online publication date: 1-Mar-2007. Narusuye K, Nakao T, Abe R, Nagatomi Y, Hirase K and Ozoe Y (2007) Molecular cloning of a GABA receptor subunit from Laodelphax striatella (Fallén) and patch clamp analysis of the homo-oligomeric receptors expressed in a Drosophila cell line, Insect Molecular Biology, 10.1111/j.1365-2583.2007.00766.x, 16:6, (723-733) Narahashi T, Zhao X, Ikeda T, Nagata K and Yeh J (2016) Differential actions of insecticides on target sites: basis for selective toxicity, Human & Experimental Toxicology, 10.1177/0960327106078408, 26:4, (361-366), Online publication date: 1-Apr-2007. Buckingham S (2006) Functional genomics: applying calcium imaging and RNA interference to Drosophila cell lines to identify new roles for gene products, Invertebrate Neuroscience, 10.1007/s10158-006-0025-z, 6:3, (141-144), Online publication date: 1-Sep-2006. Douris V, Swevers L, Labropoulou V, Andronopoulou E, Georgoussi Z and Iatrou K (2006) Stably Transformed Insect Cell Lines: Tools for Expression of Secreted and Membrane‐anchored Proteins and High‐throughput Screening Platforms for Drug and Insecticide Discovery Insect Viruses: Biotechnological Applications, 10.1016/S0065-3527(06)68004-4, (113-156), . Liu Z, Williamson M, Lansdell S, Denholm I, Han Z and Millar N (2005) From The Cover: A nicotinic acetylcholine receptor mutation conferring target-site resistance to imidacloprid in Nilaparvata lugens (brown planthopper), Proceedings of the National Academy of Sciences, 10.1073/pnas.0502901102, 102:24, (8420-8425), Online publication date: 14-Jun-2005. Buckingham S, Biggin P, Sattelle B, Brown L and Sattelle D (2005) Insect GABA Receptors: Splicing, Editing, and Targeting by Antiparasitics and Insecticides, Molecular Pharmacology, 10.1124/mol.105.015313, 68:4, (942-951), Online publication date: 1-Oct-2005. Buckingham S and Sattelle D (2005) GABA Receptors of Insects Comprehensive Molecular Insect Science, 10.1016/B0-44-451924-6/00095-8, (107-142), . Farrell P, Swevers L and Iatrou K (2005) Insect Cell Culture and Recombinant Protein Expression Systems Comprehensive Molecular Insect Science, 10.1016/B0-44-451924-6/00063-6, (475-507), . Schetz J and Shankar E (2004) Protein Expression in the Drosophila Schneider 2 Cell System , Current Protocols in Neuroscience, 10.1002/0471142301.ns0416s27, 27:1, Online publication date: 1-Apr-2004. Zhao X, Salgado V, Yeh J and Narahashi T (2003) Differential Actions of Fipronil and Dieldrin Insecticides on GABA-Gated Chloride Channels in Cockroach Neurons, Journal of Pharmacology and Experimental Therapeutics, 10.1124/jpet.103.051839, 306:3, (914-924), Online publication date: 1-Sep-2003. Ikeda T, Zhao X, Kono Y, Yeh J and Narahashi T (2003) Fipronil Modulation of Glutamate-Induced Chloride Currents in Cockroach Thoracic Ganglion Neurons, NeuroToxicology, 10.1016/S0161-813X(03)00041-X, 24:6, (807-815), Online publication date: 1-Dec-2003. Raymond V and Sattelle D (2002) Novel animal-health drug targets from ligand-gated chloride channels, Nature Reviews Drug Discovery, 10.1038/nrd821, 1:6, (427-436), Online publication date: 1-Jun-2002. Towers P and Sattelle D (2002) ADrosophila melanogaster cell line (S2) facilitates post-genome functional analysis of receptors and ion channels, BioEssays, 10.1002/bies.10178, 24:11, (1066-1073), Online publication date: 1-Nov-2002. Alix P, Grolleau F and Hue B (2002) Ca 2+ /Calmodulin-Dependent Protein Kinase Regulates GABA-Activated Cl − Current in Cockroach Dorsal Unpaired Median Neurons , Journal of Neurophysiology, 10.1152/jn.2002.87.6.2972, 87:6, (2972-2982), Online publication date: 1-Jun-2002. Narahashi T (2001) Neurophysiological Effects of Insecticides Handbook of Pesticide Toxicology, 10.1016/B978-012426260-7.50015-X, (335-351), . Watson G and Salgado V (2001) Maintenance of GABA receptor function of small-diameter cockroach neurons by adenine nucleotides, Insect Biochemistry and Molecular Biology, 10.1016/S0965-1748(00)00120-X, 31:2, (207-212), Online publication date: 1-Feb-2001. Raymond V, B. Sattelle D and Lapied B (2000) Co-existence in DUM neurones of two GluCl channels that differ in their picrotoxin sensitivity, NeuroReport, 10.1097/00001756-200008210-00018, 11:12, (2695-2701), Online publication date: 1-Aug-2000. Lansdell S and Millar N (2000) The influence of nicotinic receptor subunit composition upon agonist, α–bungarotoxin and insecticide (imidacloprid) binding affinity, Neuropharmacology, 10.1016/S0028-3908(99)00170-7, 39:4, (671-679), Online publication date: 1-Mar-2000. Grolleau F and Sattelle D (2009) Single channel analysis of the blocking actions of BIDN and fipronil on a Drosophila melanogaster GABA receptor (RDL) stably expressed in a Drosophila cell line , British Journal of Pharmacology, 10.1038/sj.bjp.0703507, 130:8, (1833-1842), Online publication date: 1-Aug-2000. Lee D, Chen C, Hsu T and Juang J (2000) A Baculovirus Superinfection System: Efficient Vehicle for Gene Transfer into Drosophila S2 Cells , Journal of Virology, 10.1128/JVI.74.24.11873-11880.2000, 74:24, (11873-11880), Online publication date: 15-Dec-2000. Lansdell S and Millar N (2000) Cloning and heterologous expression of Dα4, a Drosophila neuronal nicotinic acetylcholine receptor subunit: identification of an alternative exon influencing the efficiency of subunit assembly, Neuropharmacology, 10.1016/S0028-3908(00)00111-8, 39:13, (2604-2614), Online publication date: 1-Dec-2000. Huang Y, Williamson M, Devonshire A, Windass J, Lansdell S and Millar N (2000) Cloning, heterologous expression and co-assembly of Mpβ1, a nicotinic acetylcholine receptor subunit from the aphid Myzus persicae, Neuroscience Letters, 10.1016/S0304-3940(00)00969-1, 284:1-2, (116-120), Online publication date: 1-Apr-2000. Huang Y, Williamson M, Devonshire A, Windass J, Lansdell S and Millar N (2002) Molecular Characterization and Imidacloprid Selectivity of Nicotinic Acetylcholine Receptor Subunits from the Peach-Potato Aphid Myzus persicae, Journal of Neurochemistry, 10.1046/j.1471-4159.1999.0730380.x, 73:1, (380-389) Aydar E and Beadle D (1999) The pharmacological profile of GABA receptors on cultured insect neurones, Journal of Insect Physiology, 10.1016/S0022-1910(98)00114-0, 45:3, (213-219), Online publication date: 1-Mar-1999. Schetz J (2001) Protein Expression in the Drosophila Schneider 2 Cell System , Current Protocols in Neuroscience, 10.1002/0471142301.ns0416s08, 8:1, Online publication date: 1-Aug-1999. Yagodin S, Pivovarova N, Andrews S and Sattelle D (1999) Functional characterization of thapsigargin and agonist-insensitive acidic Ca2+stores in Drosophila melanogaster S2 cell lines, Cell Calcium, 10.1054/ceca.1999.0043, 25:6, (429-438), Online publication date: 1-Jun-1999. Wolff M and Wingate V (1998) Characterization and comparative pharmacological studies of a functional γ-aminobutyric acid (GABA) receptor cloned from the tobacco budworm,Heliothis virescens (Noctuidae:Lepidoptera), Invertebrate Neuroscience, 10.1007/BF02577690, 3:4, (305-315), Online publication date: 1-Mar-1998. Soderlund D (1997) Molecular Mechanisms of Insecticide Resistance Molecular Mechanisms of Resistance to Agrochemicals, 10.1007/978-3-662-03458-3_2, (21-56), . McCarroll L and King L (1997) Stable insect cell cultures for recombinant protein production, Current Opinion in Biotechnology, 10.1016/S0958-1669(97)80034-1, 8:5, (590-594), Online publication date: 1-Oct-1997. Rauh J, Benner E, Schnee M, Cordova D, Holyoke C, Howard M, Bai D, Buckingham S, Hutton M, Hamon A, Roush R and Sattelle D (2009) Effects of [ 3 H]-BIDN, a novel bicyclic dinitrile radioligand for GABA-gated chloride channels of insects and vertebrates , British Journal of Pharmacology, 10.1038/sj.bjp.0701215, 121:7, (1496-1505), Online publication date: 1-Jul-1997. Hosie A, Sattelle D, Aronstein K and ffrench-Constant R (1997) Molecular biology of insect neuronal GABA receptors, Trends in Neurosciences, 10.1016/S0166-2236(97)01127-2, 20:12, (578-583), Online publication date: 1-Dec-1997. Hosie A, Ozoe Y, Koike K, Ohmoto T, Nikaido T and Sattelle D (2012) Actions of picrodendrin antagonists on dieldrin-sensitive and -resistant Drosophila GABA receptors, British Journal of Pharmacology, 10.1111/j.1476-5381.1996.tb16074.x, 119:8, (1569-1576), Online publication date: 1-Dec-1996. Hosie A and Sattelle D (2012) Allosteric modulation of an expressed homo-oligomeric GABA-gated chloride channel of Drosophila melanogaster, British Journal of Pharmacology, 10.1111/j.1476-5381.1996.tb16720.x, 117:6, (1229-1237), Online publication date: 1-Mar-1996. Han K, Millar N, Grotewiel M and Davis R (1996) DAMB, a Novel Dopamine Receptor Expressed Specifically in Drosophila Mushroom Bodies, Neuron, 10.1016/S0896-6273(00)80139-7, 16:6, (1127-1135), Online publication date: 1-Jun-1996. Belelli D, Callachan H, Hill-Venning C, Peters J and Lambert J (2012) Interaction of positive allosteric modulators with human and Drosophila recombinant GABA receptors expressed in Xenopus laevis oocytes, British Journal of Pharmacology, 10.1111/j.1476-5381.1996.tb15439.x, 118:3, (563-576), Online publication date: 1-Jun-1996. BUCKINGHAM S, MATSUDA K, HOSIE A, BAYLIS H, SQUIRE M, LANSDELL S, MILLAR N and SATTELLE D (1996) Wild-type and Insecticide-resistant Homo-oligomeric GABA Receptors of Drosophila melanogaster Stably Expressed in a Drosophila Cell Line, Neuropharmacology, 10.1016/S0028-3908(96)00087-1, 35:9-10, (1393-1401), . Hosie A, Baylis H, Buckingham S and Sattelle D (2012) Actions of the insecticide fipronil, on dieldrin-sensitive and -resistant GABA receptors of Drosophila melanogaster, British Journal of Pharmacology, 10.1111/j.1476-5381.1995.tb15896.x, 115:6, (909-912), Online publication date: 1-Jul-1995. Smelt C, Sanders V, Puinean A, Lansdell S, Goodchild J, Millar N and Silman I (2021) Agonist and antagonist properties of an insect GABA-gated chloride channel (RDL) are influenced by heterologous expression conditions, PLOS ONE, 10.1371/journal.pone.0254251, 16:7, (e0254251) This Issue22 December 1994Volume 258Issue 1353 Article InformationDOI:https://doi.org/10.1098/rspb.1994.0178PubMed:7533909Published by:Royal SocietyPrint ISSN:0962-8452Online ISSN:1471-2954History: Manuscript received09/09/1994Manuscript accepted27/09/1994Published online01/01/1997Published in print22/12/1994 License:Scanned images copyright © 2017, Royal Society Citations and impact Large datasets are available through Proceedings B's partnership with Dryad