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Imaging of lysosomal pH changes with a fluorescent sensor containing a novel lysosome-locating group

2012; Royal Society of Chemistry; Volume: 48; Issue: 96 Linguagem: Inglês

10.1039/c2cc36785h

1364-548X

Hao Zhu, Jiangli Fan, Qunli Xu, Honglin Li, Jingyun Wang, Pan Gao, Xiaojun Peng,

Molecular Sensors and Ion Detection

Rlyso, a highly selective and sensitive pH sensor, can stain lysosomes with a novel lysosome-locating group, methylcarbitol. Rlyso was successfully used to detect lysosomal pH changes during apoptosis or induced by chloroquine while avoiding the "alkalizing effect" on lysosomes of current lysosomal probes with nitrogen-containing sidechains.