Requirement for FBP17 in Invadopodia Formation by Invasive Bladder Tumor Cells
2011; Lippincott Williams & Wilkins; Volume: 185; Issue: 5 Linguagem: Inglês
10.1016/j.juro.2010.12.027
ISSN1527-3792
AutoresHayato Yamamoto, Mihoko Sutoh, Shingo Hatakeyama, Yasuhiro Hashimoto, Takahiro Yoneyama, Takuya Koie, Hisao Saitoh, Kanemitsu Yamaya, Tomihisa Funyu, Toshiya Nakamura, Chikara Οhyama, Shigeru Tsuboi,
Tópico(s)Urological Disorders and Treatments
ResumoNo AccessJournal of UrologyInvestigative Urology1 May 2011Requirement for FBP17 in Invadopodia Formation by Invasive Bladder Tumor Cells Hayato Yamamoto, Mihoko Sutoh, Shingo Hatakeyama, Yasuhiro Hashimoto, Takahiro Yoneyama, Takuya Koie, Hisao Saitoh, Kanemitsu Yamaya, Tomihisa Funyu, Toshiya Nakamura, Chikara Ohyama, and Shigeru Tsuboi Hayato YamamotoHayato Yamamoto Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan , Mihoko SutohMihoko Sutoh Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Japan , Shingo HatakeyamaShingo Hatakeyama Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan , Yasuhiro HashimotoYasuhiro Hashimoto Department of Urology, Oyokyo Kidney Research Institute, Hirosaki, Japan , Takahiro YoneyamaTakahiro Yoneyama Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan , Takuya KoieTakuya Koie Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan , Hisao SaitohHisao Saitoh Department of Urology, Oyokyo Kidney Research Institute, Hirosaki, Japan , Kanemitsu YamayaKanemitsu Yamaya Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Japan , Tomihisa FunyuTomihisa Funyu Department of Urology, Oyokyo Kidney Research Institute, Hirosaki, Japan Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Japan , Toshiya NakamuraToshiya Nakamura Department of Biomedical Sciences, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Japan , Chikara OhyamaChikara Ohyama Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan , and Shigeru TsuboiShigeru Tsuboi Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan View All Author Informationhttps://doi.org/10.1016/j.juro.2010.12.027AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Invadopodia (protrusions of the plasma membrane formed by invasive tumor cells) have an essential role in bladder tumor invasion. To understand the process of bladder tumor invasion it is crucial to investigate the molecular mechanisms of invadopodia formation. We found that invasive bladder tumor cells express FBP17. In this study we examined the role of FBP17 in bladder tumor cell invadopodia formation and invasion. Materials and Methods: We used the 3 bladder tumor cell lines YTS-1, T24 and RT4 (ATCC®), and primary culture of bladder tumors from patients. Cells were stained with phalloidin for invadopodia formation. FBP17 knockdown cells were tested for invadopodia formation and subjected to invasion assay using a Transwell® cell culture chamber. We also examined the role of the extended FER-CIP4 homology and Src homology 3 domains of FBP17 in invadopodia formation in FBP17 mutant constructs. Results: Invadopodia formation was observed in invasive bladder tumor cells and FBP17 was localized to invadopodia in invasive cells. FBP17 knockdown decreased invadopodia formation in invasive cells to 13% to 14% (p <0.0005) and decreased their invasive capacity to 14% to 16% (p <0.001). The extended FER-CIP4 homology and Src homology 3 domains of FBP17 were necessary for invadopodia formation and invasion. Conclusions: Invadopodia formation requires membrane deformation activity and recruitment of dynamin-2 mediated by FBP17. FBP17 has a critical role in the process of bladder tumor cell invasion by mediating invadopodia formation. References 1 : Cancer statistics, 2009. CA Cancer J Clin2009; 59: 225. Google Scholar 2 : Cell motility through plasma membrane blebbing. J Cell Biol2008; 181: 879. Google Scholar 3 : Invadopodia: at the cutting edge of tumour invasion. J Clin Neurosci2008; 15: 725. Google Scholar 4 : Adhesions that mediate invasion. Int J Biochem Cell Biol2006; 38: 1875. Google Scholar 5 : Actin machinery and mechanosensitivity in invadopodia, podosomes and focal adhesions. J Cell Sci2009; 122: 3037. 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Google Scholar © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 5May 2011Page: 1930-1938 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.Keywordsneoplasm invasivenessurinary bladder neoplasmscell surface extensionshumanurinary bladderFNBP1 proteinAcknowledgmentsDrs. T. Miura, T. Sakurai and T. Sato, Hirosaki University Graduate School of Health Sciences, provided technical assistance and discussion. Dr. N. Mochizuki, National Cardiovascular Center Research Institute, Osaka, Japan, provided anti-FBP17 antibody. Dr. H. Kakizaki, Yamagata University, Yamagata, Japan provided the YTS-1 cell line. siRNA was synthesized at Dharmacon, Lafayette, Colorado.MetricsAuthor Information Hayato Yamamoto Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan More articles by this author Mihoko Sutoh Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Japan More articles by this author Shingo Hatakeyama Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan More articles by this author Yasuhiro Hashimoto Department of Urology, Oyokyo Kidney Research Institute, Hirosaki, Japan More articles by this author Takahiro Yoneyama Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan More articles by this author Takuya Koie Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan More articles by this author Hisao Saitoh Department of Urology, Oyokyo Kidney Research Institute, Hirosaki, Japan More articles by this author Kanemitsu Yamaya Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Japan More articles by this author Tomihisa Funyu Department of Urology, Oyokyo Kidney Research Institute, Hirosaki, Japan Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Japan More articles by this author Toshiya Nakamura Department of Biomedical Sciences, Graduate School of Health Sciences, Hirosaki University, Hirosaki, Japan More articles by this author Chikara Ohyama Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan More articles by this author Shigeru Tsuboi Department of Urology, Graduate School of Medicine, Hirosaki University, Hirosaki, Japan More articles by this author Expand All Advertisement PDF downloadLoading ...
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