Produção Nacional
Revisado por pares
Performance of PRP Associated with Porous Chitosan as a Composite Scaffold for Regenerative Medicine
2015; Hindawi Publishing Corporation; Volume: 2015; Issue: 1 Linguagem: Inglês
10.1155/2015/396131
ISSN2356-6140
AutoresAndréa Arruda Martins Shimojo, Amanda Gomes Marcelino Perez, Sofia Elisa Moraga Galdames, Isabela Cambraia de Souza Brissac, Maria Helena Andrade Santana,
Tópico(s)Tissue Engineering and Regenerative Medicine
ResumoThis study aimed to evaluate the in vitro performance of activated platelet‐rich plasma associated with porous sponges of chitosan as a composite scaffold for proliferation and osteogenic differentiation of human adipose tissue‐derived mesenchymal stem cells. The sponges were prepared by controlled freezing (−20, −80, or −196°C) and lyophilization of chitosan solutions (1, 2, or 3% w/v). The platelet‐rich plasma was obtained from controlled centrifugation of whole blood and activated with calcium and autologous serum. The composite scaffolds were prepared by embedding the sponges with the activated platelet‐rich plasma. The results showed the performance of the scaffolds was superior to that of activated platelet‐rich plasma alone, in terms of delaying the release of growth factors and increased proliferation of the stem cells. The best preparation conditions of chitosan composite scaffolds that coordinated the physicochemical and mechanical properties and cell proliferation were 3% (w/v) chitosan and a −20°C freezing temperature, while −196°C favored osteogenic differentiation. Although the composite scaffolds are promising for regenerative medicine, the structures require stabilization to prevent the collapse observed after five days.
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