Artigo Revisado por pares

A comparison of anticoagulants for the analysis of glucose concentration and for haematological measurements in bovine blood

1974; Elsevier BV; Volume: 84; Issue: 1 Linguagem: Inglês

10.1016/0021-9975(74)90027-9

ISSN

1532-3129

Autores

R. Manston, R. H. Whitlock, Emmett R. Young,

Tópico(s)

Animal Behavior and Welfare Studies

Resumo

Blood samples were taken from 18 cows into a potassium oxalate-sodium fluoride mixture, or a potassium oxalate-ammonium oxalate-ammonium fluoride mixture, or into ethylene diamine tetraacetate. Haematocrit, and the concentrations of glucose in the blood and blood plasma, were determined in each sample. Blood samples from another 6 cows were taken into the same anticoagulant mixtures and stored at 4 °C., room temperature, or 37 °C. The concentrations of glucose in the blood and plasma, haematocrit, mean corpuscular volume, erythrocyte count, and haemoglobin concentrations were determined at intervals of up to 96 h. after collection. Blood taken into the potassium oxalate-sodium fluoride mixture had reduced values for haematocrit, but there were no significant differences in haematocrit between samples taken into the other 2 anticoagulants. Little difference in the glucose concentrations of whole blood was apparent in samples taken into the 2 fluoride containing anticoagulant mixtures, but plasma concentrations of glucose were significantly lower in the samples taken into the potassium oxalate-sodium fluoride mixture. Consistent values for all determinations were obtained up to 96 h. when samples were stored at 4 °C. Some haemolysis and glycolysis occurred during storage at higher temperatures. This was more severe in the potassium oxalate-sodium fluoride mixture than in the potassium oxalate-ammonium oxalate-ammonium fluoride mixture. It is concluded that the latter is preferable for blood to be used for the determination of glucose concentrations and for haematological measurements in the metabolic profile testing of dairy herds.

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