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Online Structural Elucidation of Alkaloids and Other Constituents in Crude Extracts and Cultured Cells of Nandina domestica by Combination of LC-MS/MS, LC-NMR, and LC-CD Analyses

2008; American Chemical Society; Volume: 71; Issue: 8 Linguagem: Inglês

10.1021/np8001496

1520-6025

Kinuko Iwasa, Teturo Takahashi, Yumi Nishiyama, Masataka Moriyasu, Makiko Sugiura, Atsuko Takeuchi, Chisato Tode, Harukuni Tokuda, Kazuyoshi Takeda,

Phytochemistry and biological activities of Ficus species

The combination of NMR, MS, and CD data permitted the structural elucidation including the absolute configuration of the known alkaloids and unknown components in the extract matrix solution of Nandina domestica without isolation and sample purification prior to the coupling experiments. Unstable natural stereoisomers were identified by LC-NMR and LC-MS. Five known alkaloids, (S)-isoboldine, (S)-domesticine, (S)-nantenine, sinoacutine, and menispermine, were identified from N. domestica. O-Methylpallidine and (E, E)-, (E, Z)-, and (Z, Z)-terrestribisamide were also characterized for the first time from this plant. Known jatrorrhizine, palmatine, and berberine and unknown (R)-carnegine and (E, E)-, (E, Z)-, and (Z, Z)-terrestribisamide were identified in the callus of N. domestica.