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Rapid enumeration of viable micro-organisms by staining and direct microscopy

1989; Oxford University Press; Volume: 9; Issue: 5 Linguagem: Inglês

10.1111/j.1472-765x.1989.tb00324.x

1472-765X

Roy Betts, Pamela Bankes, J.G. Banks,

Innovative Microfluidic and Catalytic Techniques Innovation

Letters in Applied MicrobiologyVolume 9, Issue 5 p. 199-202 Rapid enumeration of viable micro-organisms by staining and direct microscopy R.P. Betts, Corresponding Author R.P. Betts Campden Food and Drink Research Association, Chipping Campden, Gloucestershire GL55 6LD, UK*Corresponding author.Search for more papers by this authorP. Bankes, P. Bankes Campden Food and Drink Research Association, Chipping Campden, Gloucestershire GL55 6LD, UKSearch for more papers by this authorJ.G. Banks, J.G. Banks Campden Food and Drink Research Association, Chipping Campden, Gloucestershire GL55 6LD, UKSearch for more papers by this author R.P. Betts, Corresponding Author R.P. Betts Campden Food and Drink Research Association, Chipping Campden, Gloucestershire GL55 6LD, UK*Corresponding author.Search for more papers by this authorP. Bankes, P. Bankes Campden Food and Drink Research Association, Chipping Campden, Gloucestershire GL55 6LD, UKSearch for more papers by this authorJ.G. Banks, J.G. Banks Campden Food and Drink Research Association, Chipping Campden, Gloucestershire GL55 6LD, UKSearch for more papers by this author First published: November 1989 https://doi.org/10.1111/j.1472-765X.1989.tb00324.xCitations: 44AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract A rapid method is described that uses the tetrazolium salt 2-(p-iodophenyl-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to stain viable micro-organisms retained on a filter membrane. Good correlation exists between numbers of INT stained cells and aerobic plate counts of single strain cultures of bacteria and yeasts. A pre-treatment step allows the technique to be used for pasteurized milk. Citing Literature Volume9, Issue5November 1989Pages 199-202 RelatedInformation