Elg1 forms an alternative RFC complex important for DNA replication and genome integrity
2003; Springer Nature; Volume: 22; Issue: 16 Linguagem: Inglês
10.1093/emboj/cdg406
ISSN1460-2075
Autores Tópico(s)Genomics and Chromatin Dynamics
ResumoArticle15 August 2003free access Elg1 forms an alternative RFC complex important for DNA replication and genome integrity Mohammed Bellaoui Mohammed Bellaoui Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Michael Chang Michael Chang Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Jiongwen Ou Jiongwen Ou Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Hong Xu Hong Xu Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Charles Boone Charles Boone Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, M5S 1A8 Canada Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Grant W. Brown Corresponding Author Grant W. Brown Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Mohammed Bellaoui Mohammed Bellaoui Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Michael Chang Michael Chang Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Jiongwen Ou Jiongwen Ou Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Hong Xu Hong Xu Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Charles Boone Charles Boone Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, M5S 1A8 Canada Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Grant W. Brown Corresponding Author Grant W. Brown Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada Search for more papers by this author Author Information Mohammed Bellaoui1, Michael Chang1, Jiongwen Ou1, Hong Xu2, Charles Boone2,3 and Grant W. Brown 1 1Department of Biochemistry, University of Toronto, Toronto, ON, M5S 1A8 Canada 2Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, M5S 1A8 Canada 3Banting and Best Department of Medical Research, University of Toronto, Toronto, ON, M5S 1A8 Canada *Corresponding author. E-mail: [email protected] The EMBO Journal (2003)22:4304-4313https://doi.org/10.1093/emboj/cdg406 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Genome-wide synthetic genetic interaction screens with mutants in the mus81 and mms4 replication fork-processing genes identified a novel replication factor C (RFC) homolog, Elg1, which forms an alternative RFC complex with Rfc2–5. This complex is distinct from the DNA replication RFC, the DNA damage checkpoint RFC and the sister chromatid cohesion RFC. As expected from its genetic interactions, elg1 mutants are sensitive to DNA damage. Elg1 is redundant with Rad24 in the DNA damage response and contributes to activation of the checkpoint kinase Rad53. We find that elg1 mutants display DNA replication defects and genome instability, including increased recombination and mutation frequencies, and minichromosome maintenance defects. Mutants in elg1 show genetic interactions with pathways required for processing of stalled replication forks, and are defective in recovery from DNA damage during S phase. We propose that Elg1-RFC functions both in normal DNA replication and in the DNA damage response. Introduction DNA replication is typically highly processive. Replication fork stalling or arrest can result when replication forks encounter damage in the DNA, and at naturally occurring sequences such as replication fork barriers and replication slow zones (Rothstein et al., 2000; Cha and Kleckner, 2002). Several mechanisms by which replication forks can be restarted following arrest have been described in bacteria (reviewed in Michel, 2000; Michel et al., 2001). Stalled forks are susceptible to breakage and to replication fork reversal, both of which generate a double-stranded DNA end. A replication fork can then be re-established by homologous recombination followed by Holliday junction resolution or by branch migration. By analogy, some of these same processes are believed to occur in eukaryotes. In addition to these pathways, which are principally involved in restarting what are presumably collapsed replication forks, recent work has demonstrated that the S phase checkpoint pathway is responsible for stabilizing replication forks and preventing fork collapse and formation of DNA structures that are substrates for replication restart pathways (Lopes et al., 2001; Tercero and Diffley, 2001; Sogo et al., 2002). Replication factor C (RFC) was first identified as a protein complex required for SV40 DNA replication in vitro (Tsurimoto and Stillman, 1989; Virshup and Kelly, 1989). RFC is a five-subunit complex that recognizes the primer terminus and catalyzes the loading of the sliding clamp proliferating cell nuclear antigen (PCNA; Hubscher, 1996; Mossi and Hubscher, 1998). PCNA acts as a processivity factor for DNA polymerase δ on the leading and lagging strands. At least two alternative forms of RFC have been identified recently in yeast and humans. In the first of these, the large subunit of RFC, Rfc1, is replaced by the Rad24 protein (Green et al., 2000). Rad24-RFC loads a PCNA-like clamp consisting of Rad17, Ddc1 and Mec3 (Bermudez et al., 2003; Majka and Burgers, 2003), is required for DNA damage checkpoint responses in G1 and G2, and contributes to S phase checkpoint responses. In the second alternative RFC, Rfc1 is replaced by Ctf18 (Hanna et al., 2001; Mayer et al., 2001; Naiki et al., 2001). Mutants in ctf18 have defects in sister chromatid cohesion (Hanna et al., 2001; Mayer et al., 2001), the process by which newly replicated chromatids remain physically associated until entry into anaphase. Ctf18-RFC also contains the Ctf8 and Dcc1 proteins, and mutations in ctf8 or dcc1 recapitulate the cohesion defects observed in ctf18 mutants (Mayer et al., 2001). There is evidence that like Rad24, Ctf18 also contributes to the S phase checkpoint response. ctf18 rad24 mutants have a mild S–M checkpoint defect that is not evident in the single mutants, and have a Rad53 activation defect in S phase (Naiki et al., 2001). Here we describe a functional genomics approach to identify previously uncharacterized factors required for the DNA damage response, particularly those involved in replication fork progression. To this end, we conducted genome-wide synthetic lethality screens with deletion mutants in mus81 and mms4. Mus81 and Mms4 are subunits of an endonuclease with a preference for branched DNA structures (Boddy et al., 2001; Chen et al., 2001; Kaliraman et al., 2001). In addition to this substrate preference, several lines of evidence connect this enzyme to the processing of stalled DNA replication forks (Haber and Heyer, 2001; Kaliraman et al., 2001; Mullen et al., 2001). We identified a previously uncharacterized RFC1 homolog, ELG1. We find that Elg1 forms an RFC-like complex with the Rfc2–5 proteins, but not with Rfc1 or its homologs Rad24 and Ctf18. ELG1 is functionally redundant with RAD24 in the DNA damage response, yet does not share its primary role in checkpoint activation. Of particular significance, elg1 mutants display defects in DNA replication in both the presence and absence of DNA damage, suggesting that Elg1 functions directly in DNA replication. Cells lacking elg1 require the intra-S phase checkpoint, homologous recombination proteins and pathways involved in replication restart following replication fork stalling for wild-type growth, and are defective in recovery from DNA damage in S phase. We propose that Elg1-RFC functions in lagging strand DNA synthesis to prevent replication fork stalling and to facilitate re-start of stalled replication forks. Results Genome-wide synthetic lethal screens with mus81Δ and mms4Δ identify ELG1 In order to identify novel genes that function to stabilize replication forks in vivo, we conducted genome-wide synthetic genetic interaction screens (Tong et al., 2001) with strains carrying deletions of MUS81 or MMS4. Several lines of evidence suggest that Mus81 and Mms4 are involved in the processing of stalled replication forks (Haber and Heyer, 2001; Kaliraman et al., 2001; Mullen et al., 2001). In these screens, the query mutant was crossed with the ∼4600 strains that make up the complete set of Saccharomyces cerevisiae viable haploid deletion mutants. Double mutants that show reduced fitness compared with single mutants, as evidenced by lack of growth or slow growth, were scored as positive. Each screen was performed three times and interactions that were scored at least twice were confirmed by tetrad analysis. The result of these screens is presented as a genetic interaction network in Figure 1A, with tetrad analysis of the elg1Δ interactions shown in Figure 1B. Each line on the network represents a synthetic lethal or synthetic sick (slow growth) interaction between the linked genes. Consistent with models in which Mus81 and Mms4 function exclusively as a heterodimer (Boddy et al., 2001; Kaliraman et al., 2001), the screen with mus81Δ identified the same set of seven genes as the screen with mms4Δ. We found strong genetic interactions between mus81Δ and mms4Δ and deletions of either sgs1 or top3. These interactions have been described previously and are believed to reflect the redundant roles of the Sgs1/Top3 and Mus81/Mms4 pathways in the repair of stalled replication forks and/or the resolution of recombination intermediates (Mullen et al., 2001). YLR235C overlaps the TOP3 gene and so was probably identified due to its effect on Top3 function. In addition to these known interactions, we identified four novel interactions, with elg1Δ, esc2Δ, nce4Δ and vid22Δ. We also found that elg1Δ is synthetic lethal with mec2-1, a checkpoint-defective allele of RAD53 (Figure 1B), which indicates that elg1Δ mutant cells require a Rad53-dependent checkpoint for viability. Together, these data suggest that Elg1 is important for the integrity of DNA replication forks in vivo. Figure 1.Genome-wide synthetic lethal screens with mus81Δ and mms4Δ identify the RFC homolog Elg1. (A) The results from synthetic genetic array analysis with mus81Δ and mms4Δ presented as a genetic interaction map. Lines connecting genes represent synthetic lethality or synthetic slow growth. Red circles indicate novel genetic interactions. (B) Tetrad confirmation of the elg1Δ crosses. Each column represents the four spores from a single ascus. Double mutant colonies, as detected by selection for the dominant selectable marker linked to each gene, are indicated by white arrowheads. (C) Schematic representation of the conserved sequence blocks in the S.cerevisiae RFC family genes. Elg1 contains six of the seven RFC boxes found in Rfc1. Download figure Download PowerPoint ELG1 is a member of the replication factor C family ELG1 (enhanced level of genome instability) was first identified in a screen for increased Ty transposon mobility, and elg1 mutants have been reported to confer an increase in direct repeat recombination (Scholes et al., 2001). Using a LEU2 direct repeat assay (Smith and Rothstein, 1999), we confirmed that deletion of elg1 causes a 7-fold increase in recombination rate (data not shown). Detailed examination of the hypothetical translation product of ELG1 revealed extensive similarity to the RFC-like protein family. Eight regions of sequence similarity have been defined in RFC proteins (Cullmann et al., 1995). As indicated in Figure 1C and in the Supplementary data (available at The EMBO Journal Online), Elg1contains all of the RFC boxes present in Rfc1 with the exception of the ligase homology region, RFC box I. The Rfc-specific boxes II, IV, VI and VIII are present in Elg1, as is the ATP-binding motif contained in boxes III and V. RFC box VII is present only in the small Rfc subunits, Rfc2–5, and is absent from Elg1. The RFC box VI in Elg1 bears greater similarity to box VIa, found in the large Rfc subunits, than it does to box VIb, found in the small subunits. Like the Rfc1 homologs Rad24 and Ctf18, Elg1 is conserved throughout Eucaryota. Homologs of Elg1 are readily identifiable in Schizosaccharomyces pombe (NP_595265), Drosophila melanogaster (AAF49530), mouse (BAC39389.1) and human (CAC44537). Together, these data indicate that Elg1 is an Rfc1 homolog and, by analogy with the Rfc1 homologs Rad24 and Ctf18, suggests that Elg1 forms an alternative RFC complex with the small RFC subunits Rfc2–5. Elg1 forms a novel RFC complex High-throughput protein–protein interaction screens have detected Elg1 in complexes with Rfc2, Rfc4 and Rfc5 (Gavin et al., 2002; Ho et al., 2002). In order to test the possibility that Elg1 forms an RFC-like complex distinct from the canonical replication RFC, we conducted co-immunoprecipitation experiments with Elg1 and the five subunits of RFC. Elg1 protein was not detectable in Rfc1 immunoprecipitates (Figure 2A, lane 4). In contrast, immunoprecipitates of Rfc2, Rfc3, Rfc4 or Rfc5 all contained Elg1 (Figure 2A, lanes 6, 8, 10 and 12). Elg1 was not immunoprecipitated in the absence of RFC, indicating that the immunoprecipitations were specific (Figure 2A, lane 2). Therefore, Elg1 forms a complex with Rfc2–5 but not with Rfc1. The simplest interpretation of these data is that Elg1 forms an alternative RFC complex in which it substitutes for Rfc1 in binding to Rfc2–5. Figure 2.Elg1 forms complexes with Rfc2, 3, 4 and 5, but not with Rfc1, Rad24 or Ctf18. (A) Extracts from yeast strains expressing the indicated epitope-tagged RFC proteins were immunoprecipitated with antibody against the flag epitope. Ten percent of the input extract (I) and the immunoprecipitate (P) were fractionated by SDS–PAGE. Immunoblots were probed with anti-flag antibody to detect Rfc1, Rfc2, Rfc3, Rfc4 and Rfc5, and with anti-myc antibody to detect Elg1. A non-specific cross-reacting polypeptide is indicated (*). (B) Extracts from strains expressing the indicated proteins were immunoprecipitated with anti-flag antibody to precipitate Rfc5 and Rad24. Immunoblots were probed with anti-flag antibody to detect Rfc5 and Rad24 and with anti-myc to detect Elg1. (C) Extracts were immunoprecipitated with anti-HA antibody to precipitate Elg1. Immunoblots were probed with anti-flag antibody to detect Rfc5, anti-myc to detect Ctf18 and anti-HA to detect Elg1. Download figure Download PowerPoint Although genetic data suggest that Elg1 is functionally distinct from Ctf18 and Rad24 as neither ctf18Δ nor rad24Δ shows as strong a genetic interaction with mus81Δ, mms4Δ or rad53 (data not shown), it remained formally possible that Elg1 is a member of the Rad24- RFC or the Ctf18-RFC complexes. To exclude these possibilities, we first immunoprecipitated Rad24 and assayed for the presence of Elg1 (Figure 2B). Elg1 was detected in Rfc5 complexes, but not in Rad24 complexes. We next immunoprecipitated Elg1 and assayed for the presence of Ctf18 (Figure 2C). Ctf18 was not present in Elg1 complexes, although again Rfc5 was present. We conclude that Elg1 forms a novel RFC complex with Rfc2–5. Elg1 function in the DNA damage response is redundant with that of Rad24 Several lines of evidence have implicated RFC complexes in the checkpoint response to DNA damage, particularly during S phase. We tested the sensitivity of elg1Δ, rad24Δ and ctf18Δ mutants, as well as all pairwise double mutants, and the triple mutant, to the DNA alkylating agent methyl methanesulfonate (MMS), the replication inhibitor hydroxyurea (HU) and UV radiation (Figure 3A). In addition, we measured mutant cell viability following exposure to 0.035% MMS in a 4 h time course (Figure 1B). First, we found that the elg1Δ mutant was only mildly sensitive to MMS, and was resistant to HU and UV. This contrasts with rad24Δ, which was significantly sensitive to all three agents, and with ctf18Δ, which displayed sensitivity to MMS and to HU, but was only modestly UV sensitive. These different sensitivities distinguish the Rfc1 homologs, and indicate that Elg1 is not a central player in DNA damage repair. Figure 3.Elg1 is required for the DNA damage response. (A) Ten-fold serial dilutions of cultures of the indicated mutants were spotted on YPD, YPD containing 0.01% (v/v) MMS, 0.035% (v/v) MMS, 50 mM HU, or on YPD that was subsequently exposed to 100 J/m2 UV. Plates were incubated at 30°C for 2–3 days. (B) Logarithmically growing cultures of the indicated mutants were incubated in YPD containing 0.035% (v/v) MMS at 30°C. At the indicated times, samples were withdrawn and plated on medium lacking MMS to determine the number of viable cells. The percentage of viable cells relative to the number of viable cells at t = 0 is shown. Plots represent the average of three experiments, and error bars span 1 SD. Download figure Download PowerPoint We next examined the sensitivity of each possible double mutant combination and the triple mutant. Both double mutants with rad24Δ were significantly more sensitive to all agents than any of the single mutants, whereas the rad24Δ ctf18Δ elg1Δ triple mutant conferred the greatest sensitivity to MMS. These findings indicate that the Rfc1 homologs are in partially redundant pathways for DNA damage resistance. Elg1 contributes to Rad53 activation Rad24 has a well-documented role in activation of the checkpoint kinase Rad53 (Shimomura et al., 1998; Pellicioli et al., 1999; Naiki et al., 2000). Activation of Rad53 can be readily assessed by immunoblot detection of a phosphorylation-dependent shift in Rad53 mobility (Pellicioli et al., 1999). We first assayed Rad53 activation in HU-arrested cells to assess S–M checkpoint function in elg1Δ cells (Figure 4). ELG1 was not required for Rad53 activation following HU arrest, as we observed wild-type levels of Rad53 phosphorylation in the elg1Δ mutant (Figure 4A). The ctf18Δ rad24Δ mutant displayed a clear defect in Rad53 activation, as previously reported (Naiki et al., 2001) and consistent with the increased HU sensitivity of this double mutant relative to the ctf18Δ and rad24Δ single mutants (Figure 3). The elg1Δ rad24Δ and elg1Δ ctf18Δ double mutants displayed slight defects in Rad53 activation, as evidenced by the smaller fraction of Rad53 present in the most slowly migrating form when compared with wild-type (Figure 4A). As with ctf18Δ rad24Δ, this might account for the increased HU sensitivity of the double mutants compared with the single mutants (Figure 3). In the elg1Δ rad24Δ ctf18Δ triple mutant, phosphorylation of Rad53 was almost completely absent (Figure 4A). We conclude that ELG1 contributes to the S–M checkpoint in the context of a rad24 mutation. The near absence of Rad53 phosphorylation in the triple mutant indicates that all of the Rfc1 homologs can contribute to Rad53 activation in S phase. Figure 4.Rad53 activation defects in elg1Δ. (A) S–M checkpoint. Logarithmically growing cultures were arrested in G1 with α-factor and released into medium containing 200 mM HU. At the indicated times, samples were fixed and extracts fractionated by SDS–PAGE. Following transfer, the immunoblot was probed with anti-Rad53 antibody. Phosphorylation of Rad53 causes a shift in electrophoretic mobility (Rad53-P) and is a marker for checkpoint activation. (B) Intra-S phase checkpoint. Logarithmically growing cultures were treated with 0.035% (v/v) MMS. At the indicated times, samples were withdrawn and Rad53 activation was analyzed by immunoblotting. (C) Cell cycle progression in the presence of MMS was assessed by flow cytometry. Logarithmically growing cultures were treated with 0.035% (v/v) MMS. At the indicated times, samples were fixed and the DNA contents of cells in each sample were analyzed by flow cytometry. The positions of cells with 1C and 2C DNA contents are indicated on the histograms. Download figure Download PowerPoint We next treated cells with MMS to assess the role of Elg1 in the intra-S phase checkpoint (Figure 4B). Again, elg1Δ cells displayed no defect in Rad53 activation. However, when combined with rad24Δ, both elg1Δ and ctf18Δ have significant Rad53 activation defects, consistent with roles in intra-S checkpoint response. It is worth noting, however, that the elg1Δ ctf18Δ double mutant had little, if any, Rad53 activation defect, indicating that Rad24 plays the more important role in the intra-S checkpoint. Since we had hypothesized a role for Elg1 in replication fork integrity, we assessed intra-S phase checkpoint function directly. Cells in asynchronous culture were treated with MMS, and their accumulation in S phase was monitored by flow cytometry (Figure 4C). Wild-type cells, with an intact intra-S phase checkpoint, accumulate in S phase by 2 h, and remain blocked with an intermediate DNA content for the 4 h duration of the experiment. This S phase accumulation is due to checkpoint-independent slowing of DNA replication fork progression combined with a checkpoint-dependent inhibition of dormant and late origin firing (Tercero and Diffley, 2001). In the rad53 mutant, the checkpoint-dependent inhibition of origin firing is abrogated and cells appeared to move through S phase more rapidly, accumulating with a 2C DNA content by 3 h. In the rad24Δ mutant, which is partially defective in the intra-S checkpoint (Paulovich et al., 1997), cells moved through S phase more rapidly than wild-type but not as rapidly as rad53 mutant cells. The elg1Δ resembled wild-type, and deleting elg1 did not enhance the intra-S checkpoint defect in rad24Δ. In contrast, the ctf18Δ rad24Δ mutant was more defective in the intra-S checkpoint, accumulating with 2C DNA content with kinetics similar to those observed with the rad53 mutant. Cells lacking Elg1 require homologous recombination and replication fork re-start pathways for optimum growth To gain further insight into Elg1 function in vivo, we performed a genome-wide synthetic lethal screen with elg1Δ. The synthetic genetic interactions confirmed by tetrad analysis are presented in Figure 5 and in the Supplementary data. Several functional clusters of genes are readily apparent. First, elg1Δ interacts with members of the RAD52 epistasis group (rad51Δ, rad52Δ, rad54Δ, rad55Δ and rad57Δ), which are required for homologous recombination. The genes rad50Δ, mre11Δ and xrs2Δ were also identified. These interactions are likely to reflect the role of these genes in homologous recombination rather than in non-homologous end joining, as elg1Δ is not synthetic lethal or sick with dnl4Δ or yku80 (data not shown). Of particular significance, genes thought to be in redundant pathways for re-starting stalled replication forks (Kaliraman et al., 2001; Mullen et al., 2001) have synthetic genetic interactions with elg1Δ. mus81Δ, mms4Δ, sgs1Δ, top3Δ, slx5Δ and slx8Δ all displayed a fitness defect in combination with elg1Δ. The last functional group of note comprises rad24Δ, rad17Δ, mec3Δ, ddc1Δ, mrc1Δ and tof1Δ. These genes are all linked to defects in S phase checkpoints (Alcasabas et al., 2001; Foss, 2001; Tanaka and Russell, 2001; Osborn et al., 2002), and their identification in the screen is consistent with lack of Elg1 causing DNA lesions or arrested replication forks during S phase. Our comprehensive genetic analysis indicates that cells lacking Elg1 and homologous recombination, replication fork re-start or S phase checkpoint pathways have a significant fitness defect, indicating that these pathways are required for optimal growth when ELG1 is deleted. Finally, the functional classes of genes identified in the elg1 synthetic genetic screen bear a striking similarity to those identified in screens with rad27Δ (Tong et al., 2001). Therefore, the genetic data suggest that Elg1 performs a function that is similar to that of Rad27, which plays an important role in Okazaki fragment maturation (Merrill and Holm, 1998; Parenteau and Wellinger, 1999). Figure 5.Genome-wide synthetic genetic screens with elg1Δ identify homologous recombination, fork re-start and S phase checkpoint pathways. The results of synthetic genetic array analysis with elg1Δ presented as a genetic interaction map. Lines connecting genes represent synthetic lethality or synthetic slow growth. Colored circles designate the cellular role of the interacting genes. Download figure Download PowerPoint Elg1 is required for replication fidelity Mutants defective in Okazaki fragment maturation, such as rfc1-1, pol30-52 and rad27Δ, all have increased forward mutation rates, but differ in the spectrum of mutants produced (Xie et al., 1999, 2001). Whereas rfc1-1 and pol30-52 cause predominantly point mutations and small insertions or deletions, rad27Δ causes large rearrangements. We assessed the forward mutation rate to canavanine resistance of elg1Δ, and found that it was almost 8-fold higher than the wild-type strain (24.2 × 10−7 versus 3.24 × 10−7), indicating that Elg1 is important for replication fidelity. To determine the spectrum of mutations caused by elg1Δ, we examined the CAN1 gene from 20 independent Canrmutants from both wild-type and elg1Δ for the presence of large insertion or deletion mutations. A difference in the size of a CAN1-derived fragment was observed in two out of 20 Canr wild-type strains, and in only one out of 20 Canr elg1Δ strains (data not shown). DNA sequencing revealed that the mutations in the elg1Δ strains were predominantly base substitution mutations, with some small (<5 bp) deletions and insertions. Therefore, the elg1Δ mutation spectrum resembles that caused by defects in Rfc1 or PCNA. elg1Δ mutants are defective in S phase progression If elg1Δ cells are defective in fork re-start or have increased levels of fork stalling, we would expect to detect defects in S phase progression. We arrested cells in G1 phase and released them synchronously into the cell cycle. Progression through S phase was measured by flow cytometry. As shown in Figure 6A, the wild-type culture completed S phase by 60 min after release, as evidenced by the accumulation of cells with a 2C DNA content. In the elg1Δ culture, however, a significant fraction of cells still had a 1C or intermediate (<2C) DNA content at 60 min. Even at 80 min post-release, some elg1Δ cells had not completed S phase. Consistent with a role for Elg1 in S phase progression, we found that elg1Δ has a probability of plasmid loss that is 7.6 times higher than wild-type in a minichromosome maintenance assay (Figure 6B). This elevated plasmid loss was not suppressed significantly by the presence of additional origins of replication, and 2D gel analysis did not reveal any initiation defects in elg1Δ (data not shown). Furthermore, we found that elg1Δ did not have any detectable defect in sister chromatid cohesion (data not shown), suggesting that plasmid loss in elg1Δ was not due to a segregation defect. Figure 6.elg1Δ mutants display DNA replication defects. (A) Progression through S phase. Wild-type or elg1Δ cells were arrested in G1 (t = 0) and released synchronously into the cell cycle. Samples were removed at the indicated times and analyzed by flow cytometry. The shaded histograms represent the cell cycle distribution of the asynchronous cultures before the G1 arrest. Overlaid histograms represent the cell cycle distribution at the indicated times after release from the G1 arrest. The positions of cells with 1C and 2C DNA contents are indicated. (B) Plasmid loss in wild-type, elg1Δ and ctf19Δ. The probability of plasmid loss per generation is plotted, and error bars span 1 SD. (C) Suppression of elg1Δ MMS sensitivity by PCNA overexpression. Serial dilutions of wild-type or elg1Δ cells carrying empty vector (v) or GAL1-POL30 plasmid (POL30) were plated on synthetic medium with 2% glucose (Glu; uninduced) or 2% galactose + 2% raffinose (Gal; induced), plus or minus 0.01% MMS. Download figure Download PowerPoint Since Elg1-RFC most probably functions as a clamp loader, we tested whether overexpression of PCNA could suppress the elg1Δ phenotype. As shown in Figure 6C, overexpression of PCNA rescued the MMS sensitivity of elg1Δ, as evidenced by improved growth on MMS plates when PCNA overexpression was induced by the presence of galactose. Taken together, these results suggest that Elg1 plays a direct role in DNA replication, most probably during the elongation phase of DNA synthesis. Abnormal recovery from replication fork stalling in elg1Δ We measured S phase progression in medium containing 0.035% MMS, a concentration of MMS known to cause extensive fork stalling in wild-type cells (Tercero and Diffley, 2001) (Figure 7A). In the presence of MMS, S phase was prolonged in the wild-type cells, which take 120 min to accumulate with 2C DNA content. The elg1Δ mutant was clearly defective in S phase progression in the presence of MMS, with a significant fraction of cells containing <2C DNA at 160 min post-release. Thus the introduction of fork stalls causes further defects in DNA synthesis in elg1 mutants, consistent with Elg1 functioning in preventing fork stalling or in re-starting stalled replication forks. Figure 7.elg1Δ mutants are d
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