Morphometric model of lymphocyte as applied to scanning flow cytometry
2006; Elsevier BV; Volume: 102; Issue: 1 Linguagem: Inglês
10.1016/j.jqsrt.2006.02.069
ISSN1879-1352
AutoresValery A. Loiko, Gennady I. Ruban, Olga A. Gritsai, А. Д. Груздев, С. М. Космачева, Н. В. Гончарова, A. A. Miskevich,
Tópico(s)Cell Image Analysis Techniques
ResumoThe peripheral blood lymphocytes of normal individuals are investigated by methods of specialized light microscopy. Lymphocytes as a whole and T-cell subpopulation are considered. Lymphocyte structure is characterized with reference to polarizing scanning flow cytometry. The lymphocyte and lymphocyte nucleus shapes are analyzed. Linear correlation dependence between sizes of lymphocyte and its nucleus is indicated. A morphometric model of a lymphocyte is constructed using the obtained data. The findings can be used, for instance, as input parameters to solve the direct and inverse light-scattering problems of turbidimetry, nephelometry, and flow cytometry.
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