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Blood glucose and liver glycogen levels in glucagon-treated chick embryos

1964; Elsevier BV; Volume: 4; Issue: 1 Linguagem: Inglês

10.1016/0016-6480(64)90032-2

1095-6840

Robert C. Thommes, Conrad E. Firling,

Reproductive Biology and Fertility

The capabilities of chick embryos incubated 6.0–18.0 days to respond to glucagon and recover from its hyperglycemic effect have been examined. A single addition of glucagon solution (Lilly) at a dosage level of 150 or 300 μg was placed on the chorioallantois of 6.0-, 8.0-, 10.0-, 12.0-, 14.0-, and 16.0-day embryos. Blood glucose samples were collected from days 8.0 through 18.0 while livers were procured from embryos sacrificed from days 6.0 through 18.0 of incubation. Liver glycogen levels in glucagon-treated chick embryos indicate that the chick is capable of responding to the hormone as early as day 6 of incubation. Both blood glucose and liver glycogen levels in treated chick embryos indicate that the magnitude and duration of response varies with the age of the embryo. The recovery of blood glucose and liver glycogen levels in the 6.0–10.0-day incubation interval demonstrates that the early chick does have the capacity to regulate blood glucose as well as liver glycogen levels to normal after the addition of a hormonal “hyperglycemic agent.”