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DNA adduct formation of benzo[a]pyrene in white blood cells of workers exposed to polycyclic aromatic hydrocarbons

2005; Elsevier BV; Volume: 208; Issue: 3 Linguagem: Inglês

10.1016/j.ijheh.2005.01.023

1618-131X

Thomas Mensing, Boleslaw Marczyński, Beate Engelhardt, Michael Wilhelm, Ralf Preuss, Martin Kappler, J. Angerer, Heiko U. Käfferlein, Michael Scherenberg, Albrecht Seidel, Thomas Brüning,

Effects and risks of endocrine disrupting chemicals

The major DNA adducts of anti-benzo[a]pyrene diolepoxide (BPDE) were determined by high performance liquid chromatography with fluorescence detection (HPLC-FLD) in white blood cells (WBC) of workers exposed to benzo[a]pyrene (B[a]P). In addition, ambient concentrations of B[a]P at the workplace were determined by personal air sampling. Workers in a refractory setting were examined before (n=26) and 3 months after (n=33) changing the production material (binding pitch). Furthermore, 9 coke oven workers were examined. The change in the production process in the refractory setting led to a decrease in the median of ambient B[a]P concentrations (0.14 to <0.07 μg/m3). The median of BPDE-DNA adduct levels in WBC also decreased from 0.9 adducts/108 nucleotides before changing the production material to <0.5 adducts/108 nucleotides 3 months afterwards. The B[a]P concentrations at the workplace for the coke oven workers were found to be significantly higher than in the refractory setting. However, BPDE-DNA adduct concentrations in coke oven workers and refractory setting workers showed no significant difference, which was probably due to the low number of studied subjects in the coke-oven setting. No significant differences could be observed for BPDE-DNA adduct levels between current smokers (n=21) and non-smokers (n=14; p=0.93) from both plants. In addition, no correlation between B[a]P concentrations in the air and DNA adduct levels in refractory workers and in coke oven workers could be found (r=-0.03, p=0.87). Because of the missing correlation between personal air sampling and BPDE-DNA adduct levels in WBC, the results may indicate that their formation is either influenced by other routes of exposure to B[a]P (e.g., skin absorption, dietary habits) or interindividual differences in their formation and repair.