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Production of a new antiserum to arthropod molting hormone and comparison with two other antisera

1981; Pergamon Press; Volume: 14; Issue: 5 Linguagem: Inglês

10.1016/0022-4731(81)90353-8

1878-2353

Cynthia Soumoff, D. H. S. Horn, John D. O’Connor,

Insect Resistance and Genetics

β-ecdysone 2-hemisuccinate was conjugated to thyroglobulin yielding 166 haptens per molecule of protein. The antiserum raised against this conjugate had a strong binding affinity for β-ecdysone and displayed reduced affinity for β-ecdysone analogs with altered hydroxyl functions along the sidechain. One exception was α-ecdysone which competed more effectively than β-ecdysone for antibody binding sites. Antiserum sensitivity toward these two hormones varied over a fifteen week interval. This antiserum and two others were used to estimate the concentration of β-ecdysone in crab hemolymph.