Homogeneous enzyme immunoassay of estradiol using estradiol-3-0-carboxymethyl ether as hapten
1988; Elsevier BV; Volume: 51; Issue: 5-6 Linguagem: Inglês
10.1016/0039-128x(88)90048-7
ISSN1878-5867
AutoresTarun K. Dhar, Ajoy K. Samanta, Esahak Ali,
Tópico(s)Viral Infectious Diseases and Gene Expression in Insects
ResumoA homogeneous enzyme immunoassay for estradiol estimation has been developed, which can be extended to other steroids. A new procedure for the preparation of estradiol -3-0- carboxymethyl ether by a simple one step reaction in high yield (90%) has been described. This hapten has been used for raising highly specific anti-estradiol antibody in rabbits and for preparation of enzyme conjugates. Two different enzymes, lysozyme and glucose -6- phosphate dehydrogenase have been studied for their suitability as enzyme labels. Our results indicate that lysozyme-conjugate meets the essential requirement for a practical enzyme immunoassay. The advantage of the present nonradioactive procedure is the overall simplicity, low cost and high stability of the reagents.
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