Protein synthesis in lymph nodes during the development of contact sensitivity in the guinea-pig
1968; Elsevier BV; Volume: 5; Issue: 1 Linguagem: Inglês
10.1016/0019-2791(68)90223-1
ISSN1878-237X
Autores Tópico(s)Signaling Pathways in Disease
Resumo3rotein synthesis in lymph nodes of non-sensitized guinea-pigs and of guinea-pigs sensitized 4 and 6 days previously to the chemical agent ‘oxazolone’ was studied by in vitro incorporation of 14C-leudine. Analysis of extracts of these lymph nodes by DEAE-cellulose chromatography and autoradio-immunoelectrophoresis showed that there was a marked increase in 14C-leudine incorporation by lymph nodes on the fourth day after sensitization and a subsequent reduction by the sixth day. Correlated with this, there was a significant increase in synthesis of γ2-globulin by 4-day sensitized lymph nodes which were also synthesizing a number of other proteins, at least 3 of which migrated electrophoretically in the α-globulin region. Radioactive peaks from DEAE-cellulose chromatography of extracts of 4-day sensitized lymph nodes were sub-fractionated by Sephadex G-200 gel-filtration. This revealed a number of newly-synthesized components which were not detectable by immunoelectrophoresis. The increased synthesis of proteins at 4 days after sensitization coincides with the presence in the lymph node of maximum numbers of immunoblasts, and the possible significance of this is discussed.
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