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Molecular organization of the nodal region is not altered in spontaneously diabetic BB‐Wistar rats

2001; Wiley; Volume: 65; Issue: 2 Linguagem: Inglês

10.1002/jnr.1137

1097-4547

Alexander Brown, Theodore Xu, Edgardo J. Arroyo, S. Rock Levinson, Peter Brophy, Elior Peles, Steven S. Scherer,

Neuroscience and Neuropharmacology Research

Abstract We examined the organization of the molecular components of the nodal region in spontaneously diabetic BB‐Wistar rats. Frozen sections and teased fibers from the sciatic nerves were immunostained for nodal (voltage‐gated Na + channels, ankyrin G , and ezrin), paranodal (contactin, Caspr, and neurofascin 155 kDa), and juxtaparanodal (Caspr2, the Shaker ‐type K + channels Kv1.1 and Kv1.2, and their associated subunit Kvβ2) proteins. All of these proteins were properly localized in myelinated fibers from rats that had been diabetic for 15–44 days, compared to age‐matched, nondiabetic animals. These results demonstrate that the axonal membrane is not reorganized, so nodal reorganization is not likely to be the cause of nerve conduction slowing in this animal model of acute diabetes. J. Neurosci. Res. 65:139–149, 2001. © 2001 Wiley‐Liss, Inc.