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Activation of α 1D Adrenergic Receptors in the Rat Urothelium Facilitates the Micturition Reflex

2005; Lippincott Williams & Wilkins; Volume: 175; Issue: 1 Linguagem: Inglês

10.1016/s0022-5347(05)00016-9

1527-3792

Hiroko Ishihama, Yoshiharu Momota, Haruko Yanase, Xiao-Jun Wang, William C. de Groat, Masahito Kawatani,

Circadian rhythm and melatonin

No AccessJournal of UrologyInvestigative urology1 Jan 2006Activation of α1D Adrenergic Receptors in the Rat Urothelium Facilitates the Micturition Reflex Hiroko Ishihama, Yoshiharu Momota, Haruko Yanase, Xiaojun Wang, William C. de Groat, and Masahito Kawatani Hiroko IshihamaHiroko Ishihama , Yoshiharu MomotaYoshiharu Momota , Haruko YanaseHaruko Yanase , Xiaojun WangXiaojun Wang , William C. de GroatWilliam C. de Groat , and Masahito KawataniMasahito Kawatani View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)00016-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Previous studies have revealed that the activation of α1 adrenergic receptors in urothelial cells releases neurotransmitters. We determined if α1D adrenergic receptors are expressed in the urothelium of the rat bladder and if inhibition of these receptors affects reflex voiding. Materials and Methods: Female Wistar rats were used in the experiments. Receptor expression was evaluated by Western blot. The effects of receptor activation were studied using cystometrograms, measurement of adenosine triphosphate concentrations in the bladder lumen and afferent nerve recording. The α1D antagonist naftopidil (0.75 to 1.66 mg/kg) was administered intravenously into the external jugular vein. Results: The expression of α1D adrenergic receptors was detected in urothelial tissue with Western blot and immunohistochemistry. The α1D receptor antagonist naftopidil prolonged the intercontraction interval during continuous infusion cystometrograms in conscious rats (143% of the control value) and suppressed the excitatory effect of intravesical infusion of acetic acid (0.1%) on the intercontraction interval (220%). Naftopidil inhibited the bladder afferent nerve activity induced by bladder distention (32.0%) and acetic acid infusion (30.4%), and decreased adenosine triphosphate levels in the bladder perfusate during bladder distention (36.6%). Conclusions: Endogenous catecholamines appear to act on α1D receptors in the urothelium to facilitate mechanosensitive bladder afferent nerve activity and reflex voiding. References 1 : Neurophysiology of micturition and its modification in animal models of human disease. In: . Edited by . London: Harwood Academic Publishers1993: 227. chapt. 8. Google Scholar 2 : Contraction of urinary bladder by central norepinephrine originating in the locus coeruleus. J Urol1988; 139: 423. Link, Google Scholar 3 : Stimulation of bladder activity by volume, L-dopa and capsaicin in normal conscious rats—effects of spinal alpha 1-adrenoceptor blockade. Naunyn Schmiedeberg Arch Pharmacol1997; 355: 787. 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Google Scholar Department of Neurophysiology, Akita University, School of Medicine, Akita, Japan and Department of Pharmacology, University of Pittsburgh, School of Medicine, Pittsburgh, Pennsylvania© 2006 by American Urological AssociationFiguresReferencesRelatedDetailsCited byMatsukawa Y, Gotoh M, Komatsu T, Funahashi Y, Sassa N and Hattori R (2018) Efficacy of Silodosin for Relieving Benign Prostatic Obstruction: Prospective Pressure Flow StudyJournal of Urology, VOL. 189, NO. 1S, (S117-S121), Online publication date: 1-Jan-2013.Kojima Y, Sasaki S, Kubota Y, Imura M, Oda N, Kiniwa M, Hayashi Y and Kohri K (2018) Up-Regulation of α1a and α1d-Adrenoceptors in the Prostate by Administration of Subtype Selective α1-Adrenoceptor Antagonist Tamsulosin in Patients With Benign Prostatic HyperplasiaJournal of Urology, VOL. 186, NO. 4, (1530-1536), Online publication date: 1-Oct-2011.Okutsu H, Matsumoto S, Ohtake A, Suzuki M, Sato S, Sasamata M and Uemura H (2018) Effect of Tamsulosin on Bladder Blood Flow and Bladder Function in a Rat Model of Bladder Over Distention/Emptying Induced Bladder OveractivityJournal of Urology, VOL. 186, NO. 6, (2470-2477), Online publication date: 1-Dec-2011.Tarcan T, Özdemir İ and Önol F (2018) The Role of α1-Adrenoceptor and Arachidonate Pathways in Increased Tone of Demucosalized Bladder TissueJournal of Urology, VOL. 181, NO. 6, (2780-2789), Online publication date: 1-Jun-2009.Matsukawa Y, Gotoh M, Komatsu T, Funahashi Y, Sassa N and Hattori R (2018) Efficacy of Silodosin for Relieving Benign Prostatic Obstruction: Prospective Pressure Flow StudyJournal of Urology, VOL. 182, NO. 6, (2831-2835), Online publication date: 1-Dec-2009.Sugaya K, Nishijima S, Tasaki S, Kadekawa K, Miyazato M and Ogawa Y (2018) Mechanisms by Which a Phytotherapeutic Drug Influences Bladder Activity in RatsJournal of Urology, VOL. 179, NO. 2, (770-774), Online publication date: 1-Feb-2008. Volume 175 Issue 1 January 2006 Page: 358-364 Advertisement Copyright & Permissions© 2006 by American Urological AssociationKeywordsnaftopidilbladderrats, Wistaradenosine triphosphatecatecholaminesMetrics Author Information Hiroko Ishihama More articles by this author Yoshiharu Momota More articles by this author Haruko Yanase More articles by this author Xiaojun Wang More articles by this author William C. de Groat More articles by this author Masahito Kawatani More articles by this author Expand All Advertisement PDF downloadLoading ...