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The Helicobacter pylori duodenal ulcer promoting gene, dupAin China

2008; BioMed Central; Volume: 8; Issue: 1 Linguagem: Inglês

10.1186/1471-230x-8-49

1471-230X

Zhiyu Zhang, Qing Zheng, Xiaoyu Chen, Shudong Xiao, Wenzhong Liu, Hong Lü,

Gastroesophageal reflux and treatments

Abstract Background The prevalence of H. pylori is as high as 60–70% in Chinese population. Although duodenal ulcer and gastric cancer are both caused by H. pylori , they are at opposite ends of the spectrum and as such are considered mutually exclusive. Duodenal ulcer promoting ( dupA ) gene was reported to be associated with duodenal ulcer development. The aim of this study was to determine the prevalence of dupA gene of Helicobacter pylori in patients with various gastroduodenal diseases and to explore the association between the gene and other virulence factors. Methods H. pylori were isolated from gastric biopsies of patients with chronic gastritis, duodenal ulcer (DU), gastric ulcer (GU), or non-cardia gastric carcinoma. The dupA , cagA , vacA , iceA and babA2 genotypes were determined by polymerase chain reaction. Histological features of gastric mucosal biopsy specimens were graded based on the scoring system proposed by the updated Sydney system. IL-1β polymorphism was investigated using restriction fragment length polymorphism. Results Isolates from 360 patients including 133 with chronic gastritis, 101 with DU, 47 with GU, and 79 with non-cardia gastric carcinoma were examined. The dupA gene was detected in 35.3% (127/360) and the prevalence DU patients was significantly greater than that in gastric cancer or GU patients (45.5% vs. 24.1% and 23.4%, P < 0.05). Patients infected with dupA -positive strains had higher scores for chronic inflammation compared to those with dupA -negative strains (2.36 vs. 2.24, p = 0.058). The presence of dupA was not associated with the cagA , vacA, iceA and babA 2 genotypes or with IL-1β polymorphisms. Conclusion In China the prevalence of dupA gene was highest in DU and inversely related to GU and gastric cancer.