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Cadmium and Zinc Binding in Mammalian Liver and Kidneys

1972; Heldref Publications; Volume: 24; Issue: 6 Linguagem: Inglês

10.1080/00039896.1972.10666118

2331-4303

Zahir A. Shaikh, O. J. Lucis,

Heavy metals in environment

Abstract Rats and mice were injected subcutaneousty with cadmium chloride Cd 109 and zinc chloride Zn 65. Both isotopes concentrated primarily in liver and kidneys. Tissue subcellular fractionation showed 70% to 80% 109Cd and 65Zn associated with cytoplasmic soluble fraction. Soluble fraction gel filtration revealed most 109Cd was bound to proteins of 11,000 to 12,000 molecular weight; 65Zn was associated mainly with larger sized molecules. In rats, two weeks following isotope dose, depletion of 65Zn from cytoplasmic macromolecules was demonstrated; 109Cd remained fixed to cadmium-binding proteins (Cd-BP). By ion-exchange chromatography, Cd-BP was resolved into major components. Similar components were separated from human kidney Cd-BP. Presence of Cd-BP was noted in humans and monkey liver tissues. It is suggested that mammalian organism intracellular Cd-BP apparently acts as biochemical mechanism for sequestration of toxic Cd2+ ions.