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BIBTEX RIS

HEMAGGLUTINATION TEST FOR THE DIAGNOSIS OF HUMAN NEUROCYSTICERCOSIS: DEVELOPMENT OF A STABLE REAGENT USING HOMOLOGOUS AND HETEROLOGOUS ANTIGENS

1997; UNIVERSIDADE DE SÃO PAULO; Volume: 39; Issue: 1 Linguagem: Inglês

10.1590/s0036-46651997000100006

ISSN

1678-9946

Autores

Andréia P. FERREIRA, Adelaide José Vaz, Paulo Mutuko Nakamura, Álvaro Toshiaki Sasaki, Antônio Walter Ferreira, J. A. Livramento,

Tópico(s)

Trace Elements in Health

Resumo

A hemagglutination (HA) test was standardized using formalin- and tannin-treated gander red blood cells sensitized with a total salt extract of C. cellulosae (HA-Cc) and an antigenic extract of Cysticercus longicollis (HA-Cl) vesicular fluid. A total of 61 cerebrospinal fluid (CSF) samples were assayed, 41 from patients with neurocysticercosis and 20 from a control group, which were, respectively, reactive and non-reactive to ELISA using C. cellulosae. The CSF samples from the control group did not react and 35 (85.4%) and 34 (82.9%) CSF samples from patients were reactive to the HA-Cc and HA-Cl tests, respectively. The reagents ready for use were stable up to 6 months when stored at 4°C in 50% glycerol. The present results confirm that the reagent using Cysticercus longicollis stabilized with glycerol can be used as an alternative in the immunological diagnosis of neurocysticercosis

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