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Electrochemical detection of kinase-catalyzed phosphorylation using ferrocene-conjugated ATP

2007; Royal Society of Chemistry; Issue: 4 Linguagem: Inglês

10.1039/b714383d

1364-548X

Haifeng Song, Kağan Kerman, Heinz‐Bernhard Kraatz,

Advanced Biosensing Techniques and Applications

Adenosine-5′-[γ-ferrocene] triphosphate is exploited as a co-substrate for the phosphorylation of the surface-immobilized peptide C-SIYRRGSRRWRKL by protein kinase C, in which the γ-ferrocene phosphate is transferred to the peptide and then detected by cyclic voltammetry.