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Specific assay for pregnancy-specific-β1-glycoprotein (SP1-β). Preparation of antiserum specific for SP1-β by absorption with a crossreacting high molecular weight serum protein

1979; Elsevier BV; Volume: 1; Issue: 3 Linguagem: Inglês

10.1016/0165-0378(79)90016-0

1872-7603

Peter Hindersson, J. Folkersen, J.G. Westergaard, B. Teisner, S.-E. Svehag,

Blood properties and coagulation

Two pregnancy-associated proteins reacting with antibody against pregnancy-specific beta 1-glycoprotein (PS beta G or SP1) were separated by means of ammonium sulphate precipitation, size chromatography and preparative zone electrophoresis. The antibody preparation was made monospecific to the beta-mobile protein by liquid phase cross-absorption of anti-SP1 immunoglobulin preparation with the isolated alpha2-mobile high molecular weight protein. The specificity of the absorbed antibody was tested in line immunoelectrophoresis, rocket immunoelectrophoresis and crossed immunoelectrophoresis.