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2,4-Nonadienal and Benzaldehyde Bioantimutagens in Fushimi Sweet Pepper ( Fushimi-Togarashi )

1999; American Chemical Society; Volume: 47; Issue: 2 Linguagem: Inglês

10.1021/jf980653n

1520-5118

Yasushi Nakamura, Emi Suganuma, Tomoaki Matsuo, Shigehisa Okamoto, Kenji Sato, Kozo Ohtsuki,

Biochemical Analysis and Sensing Techniques

Fushimi sweet pepper, "Fushimi-togarashi", is one of the "Kyo-yasai", traditional vegetables, in Kyoto, Japan. The chloroform fraction of Fushimi sweet pepper showed bioantimutagenicity on UV induced mutation in Escherichia coli B/r WP2. The bioantimutagen was purified with silica gel chromatography and identified as 2,4-nonadienal (ID50 = 20 μg/plate) on the basis of GC retention time and EI-MS spectrum of authentic 2,4-nonadienal. The sweet pepper also contained a known bioantimutagen, benzaldehyde (ID50 = 2 mg/plate). Additive bioantimutagenicity was also observed by 2,4-nonadienal with benzaldehyde. 2,4-Nonadienal did not show bioantimutagenicity in an UV excision repair deficient strain, E. coli B/r WP2s uvrA-. Furthermore no delay of the first cell division after UV irradiation was observed in E. coli B/r WP2. These results indicate that the bioantimutagenic activity of 2,4-nonadienal on UV mutagenesis might depend on the excision repair system in E. coli B/r WP2. Keywords: 2,4-Nonadienal; benzaldehyde; antimutagen; vegetable; sweet pepper; additive effect