EFFECT OF INTRAVESICAL CAPSAICIN AND VEHICLE ON BLADDER INTEGRITY IN CONTROL AND SPINAL CORD INJURED RATS
1998; Lippincott Williams & Wilkins; Volume: 159; Issue: 3 Linguagem: Inglês
10.1016/s0022-5347(01)63838-2
ISSN1527-3792
AutoresDOLORES SHUPP BYRNE, Akhil Das, John Sedor, Bin Huang, David A. Rivas, HUGH J. FLOOD, William DeGroat, Mark L. Jordan, Michael B. Chancellor, Peter McCue,
Tópico(s)Pelvic floor disorders treatments
ResumoNo AccessJournal of UrologyInvestigative Urology1 Mar 1998EFFECT OF INTRAVESICAL CAPSAICIN AND VEHICLE ON BLADDER INTEGRITY IN CONTROL AND SPINAL CORD INJURED RATS DOLORES SHUPP BYRNE, AKHIL DAS, JOHN SEDOR, BIN HUANG, DAVID A. RIVAS, HUGH J. FLOOD, WILLIAM DEGROAT, MARK L. JORDAN, MICHAEL B. CHANCELLOR, and PETER MCCUE DOLORES SHUPP BYRNEDOLORES SHUPP BYRNE , AKHIL DASAKHIL DAS , JOHN SEDORJOHN SEDOR , BIN HUANGBIN HUANG , DAVID A. RIVASDAVID A. RIVAS , HUGH J. FLOODHUGH J. FLOOD , WILLIAM DEGROATWILLIAM DEGROAT , MARK L. JORDANMARK L. JORDAN , MICHAEL B. CHANCELLORMICHAEL B. CHANCELLOR , and PETER MCCUEPETER MCCUE View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)63838-2AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: To determine the acute effect of intravesical capsaicin on bladder mucosal integrity in normal and spinal cord injured (SCI) rats. Materials and Methods: Intravesical reagents were instilled in 5 groups of age and weight matched female rats: 1) control+normal saline solution (NSS), 2) control+ethanol (EtOH), 3) control+capsaicin/EtOH, 4) SCI+NSS, 5) SCI+capsaicin/EtOH. Intravesical instillations were performed 4 weeks after a standard T10 SCI. Intravesical capsaicin (1 mM.) was dissolved in 30% EtOH/NSS. The animals (n = 3 each group) were sacrificed at 30 minutes, 24 hours, 72 hours, and 7 days after intravesical instillation. Whole bladders were harvested, fixed in 10% buffered formalin, and paraffin embedded. Tissue blocks were blind coded and sectioned (5 micro m. thickness) for histopathological analysis. All sections were initially stained with hematoxylin and eosin (H & E). Specific staining for mucin carbohydrate moieties included periodic acid-Schiff (PAS) and alcian blue. Also, immunohistochemical staining for GP51 (a urinary glycoprotein) was performed. Results: Control and SCI rats exhibited similar bladder mucosal histology by H & E and mucin specific stains. Instillation of saline demonstrated no effect on bladder histology, whereas instillation of intravesical capsaicin induced a profound acute effect of thinning of the epithelium, submucosal edema, and diminished presence of GP51. EtOH produced similar pathological findings, but to a lesser degree than capsaicin. Intravesical capsaicin demonstrated a similar effect in both control and SCI animals. The peak effect was seen after 30 minutes and continued for 24 hours. Partial recovery was noted after 72 hours and complete recovery was evident by 1 week. Conclusions: The control and SCI rats demonstrated a histologically similar mucosa and glycosaminoglycan layer. The effect of saline instillation on the mucosa was negligible. Intravesical capsaicin dissolved in 30% ethanol/NSS had a profound effect on the bladder urothelium submucosa that was more pronounced than that seen with the ethanol vehicle alone in normal animals. References 1 : Intravesical capsaicin for treatment of detrusor hyperreflexia. Neurourol. Urodynam1992; 11: 465. Google Scholar 2 : Pharmacologically-induced selective degeneration of chemoreceptive primary sensory neurons. Nature1977; 270: 741. Google Scholar 3 : The neuropharmacology of capsaicin: review of some recent observations. Pharmacol. Rev.1986; 38: 179. Google Scholar 4 : Capsaicin-induced depletion of substance P from primary sensory neurons. Brain Res.1978; 152: 183. Google Scholar 5 : Urinary tract glycoprotein: tissue distribution and antigenic specificity. World J. Urol.1994; 12: 21. Google Scholar 6 : Antibacterial activity of bladder surface mucin duplicated in the rabbit by exogenous glycosaminoglycan (sodium pentosanopolysulfate). Infect. Immun.1980; 27: 876. Google Scholar 7 : Functional and structural characteristics of the glycosaminoglycans of the bladder luminal surface. J. Urol.1987; 138: 433. Link, Google Scholar 8 : A methodology for the characterization of urinary glycosaminoglycans. J. Urol.1984; 131: 995. Link, Google Scholar 9 : Bladder surface glycosaminoglycans: an epithelial permeability barrier. J. Urol.1990; 143: 139. Link, Google Scholar 10 : Bladder mucin: a scanning electron microscopy study in experimental cystitis. J. Urol.1982; 128: 1060. Link, Google Scholar 11 : Intravesical capsaicin for treatment of detrusor hyperreflexia. J. Neurol. Neurosurg. Psychiatry1994; 57: 169. Google Scholar 12 : Neurophysiology of micturition and its modification in animal models of human disease. In: Nervous Control of the Urogenital System. Edited by . : Harwood Academic Publishers1993: 227. Google Scholar 13 : Mechanism underlying the recovery of urinary bladder function following spinal cord injury. J. Auton. Nerv. Syst.1990; 30: 571. Google Scholar 14 : Intravesical capsaicin in neurologic impaired patients with detrusor hyperreflexia. J. Spinal Cord Med.1996; 19: 190. Google Scholar 15 : Histological and histochemical uses of periodic acid. Stain Tech.1948; 23: 99. Google Scholar 16 : Specific staining of sulphate groups with alcian blue at low pH. J. Histochem. Cytochem.1964; 12: 309. Google Scholar 17 : Distribution in human tissues of an anti-bacterial urinary glycoprotein. J. Urol.1993; 149: 405A. Google Scholar 18 : Micturition patterns after spinal trauma as a measure of autonomic functional recovery. J. Urol.1994; 151: 250. Link, Google Scholar 19 : Treatment of chronic postherpetic neuralgia with topical capsaicin. J. Am. Acad. Dermatol.1987; 17: 93. Google Scholar 20 : Postherpetic neuralgia and topical capsaicin. Pain1988; 33: 333. Google Scholar 21 : A randomized vehicle-controlled trial of topical capsaicin in the treatment of post herpetic neuralgia. Clin. Ther.1993; 15: 510. Google Scholar 22 : Safety of topical capsaicin in diabetic neuropathy. Neurology1990; 40: 160. Google Scholar 23 : Treatment of oral postherpetic neuralgia with topical capsaicin. Int. J. Dermatol.1988; 27: 336. Google Scholar 24 : Protective action of ruthenium red toward capsaicin densensitization of sensory fibers. Neurosci. Lett.1988; 88: 201. Google Scholar 25 : Immunochemical detection of capsaicin congeners. Soc. Neurosci. Abstr.1987; 13: 1409. Google Scholar 26 : Capsaicin and primary afferent neurons: from basic science to human therapy. J. Autonom. Nerv. Sys.1991; 33: 1. Google Scholar 27 : Functional properties of spinal visceral afferents supplying abdominal and pelvic organs with special emphasis on visceral nocioception. Prog. Brain Res.1986; 67: 87. Google Scholar 28 : Activation of unmyelinated afferent fibres by mechanical stimulation and inflammation of the urinary bladder in the cat. J. Physiol.1990; 425: 545. Google Scholar 29 : A bladder-to-bladder cooling reflex in the cat. J. Physiol.1990; 427: 281. Google Scholar 30 : Lessons learned from 44 intravesical instillations of capsaicin. Neurourol. Urodynam.1994; 13: 348. Google Scholar 31 : Relief of pain following intravesical capsaicin in patients with hypersensitive disorders of the lower urinary tract. Br. J. Urol.1993; 71: 686. Google Scholar 32 : Intravesical capsaicin for neurogenic bladder dysfunction. Lancet1992; 339: 1239. Google Scholar From the Departments of Urology and Pathology, Thomas Jefferson University, Philadelphia, and the Division of Urologic Surgery, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.© 1998 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 159Issue 3March 1998Page: 1074-1078 Advertisement Copyright & Permissions© 1998 by American Urological Association, Inc.MetricsAuthor Information DOLORES SHUPP BYRNE More articles by this author AKHIL DAS More articles by this author JOHN SEDOR More articles by this author BIN HUANG More articles by this author DAVID A. RIVAS More articles by this author HUGH J. FLOOD More articles by this author WILLIAM DEGROAT More articles by this author MARK L. JORDAN More articles by this author MICHAEL B. CHANCELLOR More articles by this author PETER MCCUE More articles by this author Expand All Advertisement PDF DownloadLoading ...
Referência(s)