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The use of spa and phage typing for characterization of a MRSA population in a Belgian hospital: Comparison between 2002 and 2007

2009; Elsevier BV; Volume: 58; Issue: 1 Linguagem: Inglês

10.1016/j.patbio.2009.07.024

1768-3114

C. Wildemauwe, D. De Brouwer, C. Godard, P. Buyssens, Joris de Wit, R. Joseph, R. Vanhoof,

Pneumonia and Respiratory Infections

Strain typing of pathogens is essential to pinpoint the sources and routes of transmission and to forecast future trends. In a general hospital, we studied possible changes in the MRSA population. MRSA isolates received from a Belgian general hospital, during 2002 (n = 150) and the second half of 2007 (n = 105), were compared by phage and spa typing. In 2002, [J]* phage types characterized 45% of the MRSA isolates, 13% belonged to the [O]* phage types, 12% to a local phage type 29/42E/54/D11* and 28% were not assigned to a defined group. Thirteen different spa types were found among the isolates: 39% belonged to t038, 27% to t121, 14% to t041, 5% to t740, and 4% to t002 and t024 each. Two spa types were found respectively in two and three isolates, five were unique. In 2007, 35% belonged to [J]*, 23% to [O]* and 39% could not be put in a defined group. Eighteen different spa types were found: 30% belonged to t740, 29% to t121, 13% to t038 and 10% to t002. Three spa types were represented in two isolates, eleven were unique. The t041 spa type was specific for the 29/42E/54/D11* and the majority of the t121 isolates were related to [J]*. [J]* remained the dominant phage types group but decreased whereas [O]*, the second phage types group, increased. As to the spa types, t740 became dominant while t121 remained second. Phage and spa typing point to some quantitative changes among the Belgian MRSA population. Le typage des pathogènes est essentiel dans la détection des sources et des voies de transmission comme dans l’estimation des tendances évolutives. Nous avons examiné les changements survenus chez Staphylococcus aureus méticillino-résistant (MRSA) dans un hôpital général belge. Les MRSA, isolés pendant l’année 2002 (n = 150) et la deuxième partie de l’année 2007 (n = 105), ont été comparés par lysotypie et séquençage spa. En 2002, 45 % des MRSA appartenaient aux lysotypes [J]*, 13 % aux lysotypes [O]*, 12 % à un lysotype local 29/42E/54/D11* et 28% à aucun groupe défini. On obtenait 13 types spa: t038 pour 39 %, t121 pour 27 %, t041 pour 14 %, t740 pour 5 %, t002 et t024 chacun pour 4 %, sept types ne concernaient que trois, deux ou un isolat. En 2007, 37 % des isolats appartenaient à [J]*, 23 % à [O]* et 39 % à aucun groupe défini. On obtenait 18 types spa: t740 pour 30 %, t121 pour 29 %, t038 pour 13 %, t002 pour 10 % et 14 types concernaient deux ou un isolat. Le type spa t041 était spécifique du lysotype 29/42E/54/D11* et le type spa t121 correspondait majoritairement au groupe [J]*. [J]* est resté dominant mais a diminué alors que [O]* augmentait. Le type spa t740 est devenu dominant, le type t121 est resté second. La lysotypie comme le typage spa montrent des variations quantitatives parmi les types majeurs au sein de la population de MRSA belges considérée.