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Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440

2002; Wiley; Volume: 4; Issue: 12 Linguagem: Inglês

10.1046/j.1462-2920.2002.00366.x

1462-2920

William Nelson, Christian Weinel, Ian T. Paulsen, Robert J. Dodson, H. Hilbert, Vítor A. P. Martins dos Santos, Derrick E. Fouts, Steven R. Gill, Mihai Pop, Megan E. Holmes, Lauren Brinkac, Maureen J. Beanan, Robert T. DeBoy, S. Daugherty, James F. Kolonay, Ramana Madupu, William Nelson, Owen White, J. Peterson, Hoda Khouri, Ioana R. Hance, P. Chris Lee, Erik Holtzapple, David J. Scanlan, Khiem Tran, A. Moazzez, Teresa R. Utterback, Michael Rizzo, K. Lee, D. Kosack, D. Moestl, Holger Wedler, Jürgen Lauber, D. Stjepandic, Jörg D. Hoheisel, M. Straetz, Sabina Heim, Claudia Kiewitz, Jonathan A. Eisen, Kenneth N. Timmis, Andreas Düsterhöft, Burkhard Tümmler, Claire M. Fraser,

Plant Pathogenic Bacteria Studies

Summary Pseudomonas putida is a metabolically versatile saprophytic soil bacterium that has been certified as a biosafety host for the cloning of foreign genes. The bacterium also has considerable potential for biotechnological applications. Sequence analysis of the 6.18 Mb genome of strain KT2440 reveals diverse transport and metabolic systems. Although there is a high level of genome conservation with the pathogenic Pseudomonad Pseudomonas aeruginosa (85% of the predicted coding regions are shared), key virulence factors including exotoxin A and type III secretion systems are absent. Analysis of the genome gives insight into the non‐pathogenic nature of P. putida and points to potential new applications in agriculture, biocatalysis, bioremediation and bioplastic production.