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Dietary l -Arginine Reduces the Progression of Atherosclerosis in Cholesterol-Fed Rabbits

1997; Lippincott Williams & Wilkins; Volume: 96; Issue: 4 Linguagem: Inglês

10.1161/01.cir.96.4.1282

1524-4539

Rainer H. Böger, Stefanie M. Bode‐Böger, Ralf P. Brandes, Laddaval Phivthong-ngam, M. Böhme, Reinhold Nafe, Andreas Mügge, Jürgen C. Frölich,

Hormonal Regulation and Hypertension

Background We investigated whether l -arginine induces regression of preexisting atheromatous lesions and reversal of endothelial dysfunction in hypercholesterolemic rabbits, whether similar effects can be obtained by cholesterol-lowering therapy with lovastatin, and which mechanism leads to these effects. Methods and Results Rabbits were fed 1% cholesterol for 4 weeks and 0.5% cholesterol for an additional 12 weeks. Two groups of cholesterol-fed rabbits were treated with l -arginine (2.0% in drinking water) or lovastatin (10 mg/d) during weeks 5 through 16. Systemic nitric oxide (NO) formation was assessed as the urinary excretion rates of nitrate and cGMP in weekly intervals. Cholesterol feeding progressively reduced urinary nitrate excretion to ≈40% of baseline ( P <.05) and increased plasma concentrations of asymmetrical dimethylarginine (ADMA), an endogenous NO synthesis inhibitor. Dietary l -arginine reversed the reduction in plasma l -arginine/ADMA ratio and partly restored urinary excretion of nitrate and cGMP (each P <.05 vs cholesterol) but did not change plasma cholesterol levels. l -Arginine completely blocked the progression of carotid intimal plaques, reduced aortic intimal thickening, and preserved endothelium-dependent vasodilator function. Lovastatin treatment reduced plasma cholesterol by 32% but did not improve urinary nitrate or cGMP excretion or endothelium-dependent vasodilation. Lovastatin had a weaker inhibitory effect on carotid plaque formation and aortic intimal thickening than l -arginine. l -Arginine inhibited but lovastatin potentiated superoxide radical generation in the atherosclerotic vascular wall. Conclusions Dietary l -arginine improves NO-dependent vasodilator function in cholesterol-fed rabbits and completely blocks the progression of plaques via restoration of NO synthase substrate availability and reduction of vascular oxidative stress. Lovastatin treatment has a weaker inhibitory effect on the progression of atherosclerosis and no effect on vascular NO elaboration, which may be due to its stimulatory effect on vascular superoxide radical generation.