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Amoeboid T lymphocytes require the septin cytoskeleton for cortical integrity and persistent motility

2008; Nature Portfolio; Volume: 11; Issue: 1 Linguagem: Inglês

10.1038/ncb1808

1476-4679

Aaron J. Tooley, Julia Gilden, Jordan Jacobelli, Peter Beemiller, William S. Trimble, Makoto Kinoshita, Matthew F. Krummel,

Plant and fungal interactions

Septins are cytoskeletal proteins that form a ring at the cytokinetic furrow. Now an analogous 'molecular corset' of septins is found to be required for T lymphocyte migration. The systems that refine actomyosin forces during motility remain poorly understood. Septins assemble on the T-cell cortex and are enriched at the mid-zone in filaments. Septin knockdown causes membrane blebbing, excess leading-edge protrusions and lengthening of the trailing-edge uropod. The associated loss of rigidity permits motility, but cells become uncoordinated and poorly persistent. This also relieves a previously unrecognized restriction to migration through small pores. Pharmacologically rigidifying cells counteracts this effect, and relieving cytoskeletal rigidity synergizes with septin depletion. These data suggest that septins tune actomyosin forces during motility and probably regulate lymphocyte trafficking in confined tissues.