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Application of Preparative High-Speed Countercurrent Chromatography for Separation of Elatine from Delphinium shawurense

2008; Taylor & Francis; Volume: 31; Issue: 19 Linguagem: Inglês

10.1080/10826070802424956

1520-572X

Yi Yang, Dongyu Gu, Hankui Wu, Haji Akber Aisa, Tianyou Zhang, Yoichiro Ito,

Plant-based Medicinal Research

Abstract Preparative separation of elatine in Delphinium shawurense was achieved for the first time using high speed countercurrent chromatography (HSCCC). The separation was performed with a solvent system composed of ethyl acetate-chloroform-methanol-water (3:0.1:2:3, v/v) using the lower organic phase as a mobile phase under a revolution speed of 800 rpm. This yielded 72 mg of elatine at over 97% purity with an approximately 95% recovery. The chemical structure was identified by MS and NMR. Keywords: Delphinium Shawurense ElatineHigh speed countercurrent chromatography (HSCCC) ACKNOWLEDGMENTS This work was financially supported by grant (code: KGC X2-SW-213–08) from Key Project of Knowledge Innovation Program of Chinese Academy of Sciences and grant (code: 200515123) from the program for development and research of high technologies in Xinjiang.