The measurement of acid mucopolysaccharides (glycosaminoglycans) in amniotic fluid and urine
1973; Elsevier BV; Volume: 45; Issue: 1 Linguagem: Inglês
10.1016/0009-8981(73)90147-2
ISSN1873-3492
AutoresDenise Duncan, R. W. Logan, M.A. Ferguson‐Smith, Frances Hall,
Tópico(s)Neonatal Respiratory Health Research
ResumoThe purpose of the investigation was to find a routine laboratory method potentially suitable for the prenatal detection of the mucopolysaccharidoses. Samples of amniotic fluid from therapeutic abortions were treated with pronase, dialysed and the glycosaminoglycans precipitated by cetyl pyridinium chloride (CPC). The precipitates were subjected to quantitative determinations of hexuronic acid and neutral sugar, and the glycosaminoglycans were separated qualitatively by electrophoresis on cellulose acetate and differential staining in alcian blue-magnesium chloride mixtures. The same techniques were applied to routine urine samples. Evidence is presented to support the contention that these combined investigations offer a high probability of detecting abnormalities in glycosaminoglycan metabolism.
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