Development of Stable Phosphohistidine Analogues
2010; American Chemical Society; Volume: 132; Issue: 41 Linguagem: Inglês
10.1021/ja104393t
ISSN1943-2984
AutoresJung‐Min Kee, Bryeanna Villani, Laura Rocco Carpenter, Tom W. Muir,
Tópico(s)RNA and protein synthesis mechanisms
ResumoProtein phosphorylation is one of the most common and extensively studied posttranslational modifications (PTMs). Compared to the O-phosphorylation of Ser, Thr, and Tyr residues, our understanding of histidine phosphorylation is relatively limited, particularly in higher eukaryotes, due to technical difficulties stemming from the intrinsic instability and isomerism of phosphohistidine (pHis). We report the design and synthesis of stable and nonisomerizable pHis analogues. These pHis analogues were successfully utilized in solid-phase peptide synthesis and semi-synthesis of histone H4. Significantly, the first antibody that specifically recognizes pHis was obtained using the synthetic peptide as the immunogen.
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