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Direct esterification method for analysis of long-chain polyunsaturated fatty acids (LC-PUFAs) in infant formulae

1997; Volume: 204; Issue: 1 Linguagem: Inglês

10.1007/s002170050031

1431-4649

Bertold Bohnert, Marcel Braun, H. Winter, Brigitte Flück,

Fatty Acid Research and Health

Direct esterification of lipids in foods, including liquid and powder infant formulae, can be used as a simplified analytical procedure for the analysis of long-chain polyunsaturated fatty acids. The new method gives comparable results to standard methods using lipid extraction, but with less effort. The gas chromatographic separation method described uses an internal standard to calculate not only the normalised amount of fatty acid/total fatty acids, but also the absolute amounts of fatty acids in the product.