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Antimutagenic Constituents from the Thorns of Gleditsia sinensis

2005; Pharmaceutical Society of Japan; Volume: 53; Issue: 5 Linguagem: Inglês

10.1248/cpb.53.561

1347-5223

Jae-Chul Lim, Jong Hee Park, Miloš Buděšı́nský, Alexander Kasal, Yeong-Hwan Han, Byung-Soo Koo, Seung-Il Lee, Dong-Ung Lee,

Medicinal plant effects and applications

Antimutagenic activity-guided fractionation of an extract prepared from the thorns of Gleditsia sinensis LAM. led to the isolation of one triterpenoid and four steroids, which were identified as D:C-friedours-7-en-3-one (1), stigmast-4-ene-3,6-dione (2), stigmastane-3,6-dione (3), stigmasterol (4), and β-sitosterol (5). Triterpenoid 1 was found for the first time in a natural source and the steroids 2—5 were first isolated from this plant. Stigmasterol was the most active antimutagen, showing 51.2% and 64.2% reduction of the induction factor against the mutagens MNNG and NQO, respectively, in the SOS chromotest. Some NMR data of the steroids 2 and 3 obtained have to be revised.