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Generation of Recombinant Influenza Virus from Plasmid DNA

2010; MyJOVE; Issue: 42 Linguagem: Inglês

10.3791/2057

1940-087X

Luis Martínez‐Sobrido, Adolfo García‐Sastre,

Viral gastroenteritis research and epidemiology

Efforts by a number of influenza research groups have been pivotal in the development and improvement of influenza A virus reverse genetics. Originally established in 1999 1,2 plasmid-based reverse genetic techniques to generate recombinant viruses have revolutionized the influenza research field because specific questions have been answered by genetically engineered, infectious, recombinant influenza viruses. Such studies include virus replication, function of viral proteins, the contribution of specific mutations in viral proteins in viral replication and/or pathogenesis and, also, viral vectors using recombinant influenza viruses expressing foreign proteins 3.