Monocyte-Plasmablast Crosstalk during Dengue
2014; Cell Press; Volume: 16; Issue: 1 Linguagem: Inglês
10.1016/j.chom.2014.06.011
ISSN1934-6069
Autores Tópico(s)HIV Research and Treatment
ResumoDengue virus infection induces a dramatic expansion of B cell plasmablasts. In this issue, Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar begin with transcriptomic analysis and then integrate studies in human clinical samples, nonhuman primates, and coculture of primary human cells to identify a role for CD14+CD16+ monocytes in generating plasmablast responses during dengue virus infection. Dengue virus infection induces a dramatic expansion of B cell plasmablasts. In this issue, Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar begin with transcriptomic analysis and then integrate studies in human clinical samples, nonhuman primates, and coculture of primary human cells to identify a role for CD14+CD16+ monocytes in generating plasmablast responses during dengue virus infection. Dengue virus (DENV) continues to expand as a major global health problem, with up to 100 million dengue cases per year. The four DENV serotypes (DENV1–4) are transmitted by Aedes mosquitoes and can result in a self-limiting, but debilitating, acute febrile illness (dengue fever) or the more severe and potentially life-threatening plasma leakage syndrome, dengue hemorrhagic fever/dengue shock syndrome. The primary risk factor for developing severe disease is a secondary infection with a different DENV serotype than that causing the previous infection. During acute DENV infection, a dramatic expansion of plasmablasts occurs (Wrammert et al., 2012Wrammert J. Onlamoon N. Akondy R.S. Perng G.C. Polsrila K. Chandele A. Kwissa M. Pulendran B. Wilson P.C. Wittawatmongkol O. et al.J. Virol. 2012; 86: 2911-2918Crossref PubMed Scopus (176) Google Scholar, Zompi et al., 2012Zompi S. Montoya M. Pohl M.O. Balmaseda A. Harris E. PLoS Negl. Trop. Dis. 2012; 6: e1568Crossref PubMed Scopus (78) Google Scholar). However, it is not known what factors play a role in this expansion. During secondary DENV infection, these plasmablasts demonstrate a greater cross-reactive response to DENV serotype(s) from a previous infection than to the current infecting serotype (Zompi et al., 2012Zompi S. Montoya M. Pohl M.O. Balmaseda A. Harris E. PLoS Negl. Trop. Dis. 2012; 6: e1568Crossref PubMed Scopus (78) Google Scholar). It is thought that poorly neutralizing serotype-cross-reactive antibodies can enhance viral uptake through Fcγ receptors found on DENV target cells such as macrophages and monocytes through the mechanism of antibody-dependent enhancement, which, along with cross-reactive T cells and host genetic factors, contributes to dengue disease severity. However, the antibody and T cell response can also play a protective role. What tips the balance of the adaptive immune response toward protection versus pathogenesis is an area of active investigation. In addition, it is not well understood which innate immune factors contribute to control of viral load and disease severity. In this issue of Cell Host & Microbe, Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar demonstrate how a particular subset of monocytes modulates humoral responses during DENV infection and thus unveil an important link between the innate and adaptive immune response. The authors utilize an integrated systems biology approach in which they combine results from studies in human populations, nonhuman primates, and in vitro analyses. First, they performed transcriptomic analysis of samples from Thai patients with either dengue fever or dengue hemorrhagic fever. Transcriptional signatures from these patients during DENV infection correlated with viral load and duration of illness, but not disease severity—likely because the study population did not include patients with the most severe disease, dengue shock syndrome. Previous reports have associated changes in transcriptional profile in dengue with the occurrence of shock (Loke et al., 2010Loke P. Hammond S.N. Leung J.M. Kim C.C. Batra S. Rocha C. Balmaseda A. Harris E. PLoS Negl. Trop. Dis. 2010; 4: e710Crossref PubMed Scopus (48) Google Scholar, Simmons et al., 2007Simmons C.P. Popper S. Dolocek C. Chau T.N. Griffiths M. Dung N.T. Long T.H. Hoang D.M. Chau N.V. Thao T.T. et al.J. Infect. Dis. 2007; 195: 1097-1107Crossref PubMed Scopus (144) Google Scholar). Dengue patients experience high viral load early after symptom onset; DENV viremia decreases over the course of illness so that samples taken at later time points (≥6 days) have low viral load. Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar conducted parallel analyses to evaluate gene expression signatures in patients with high versus low viral load and in samples collected early versus late after symptom onset, and overall they observed similar patterns in samples with high viral load early in disease versus low viral load late in disease progression. These findings are consistent with previous studies in which gene expression patterns exhibited temporal changes over the course of illness (Popper et al., 2012Popper S.J. Gordon A. Liu M. Balmaseda A. Harris E. Relman D.A. PLoS Negl. Trop. Dis. 2012; 6: e1966Crossref PubMed Scopus (43) Google Scholar, Sun et al., 2013Sun P. García J. Comach G. Vahey M.T. Wang Z. Forshey B.M. Morrison A.C. Sierra G. Bazan I. Rocha C. et al.PLoS Negl. Trop. Dis. 2013; 7: e2298Crossref PubMed Scopus (55) Google Scholar). Interestingly, when Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar focused only on early time points post-symptom onset and compared transcriptomic analysis in patients with high or low viral load, they found that viral load can influence gene expression patterns independently of time post-onset of illness, an observation worthy of future study. Samples with high viremia and/or collected early after symptom onset positively correlated with innate immune responses associated with type I interferon genes, whereas genes involved in cell cycle, proliferation, and metabolism correlated with lower viremia and later stages of illness. Gene set enrichment analysis detected genes associated with monocytes, dendritic cells (DCs), and macrophages in patients with high viremia early in illness, whereas genes related to natural killer cells, B cells, and CD4+ and CD8+ T cells were associated with low viremia later in the course of disease. Thus, samples with high viremia from early after symptom onset expressed genes associated with innate immune sensing of viruses and inflammatory responses to viral infection. Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar next examined alterations in blood cells during acute DENV infection and found an increased frequency of monocytes and a reduction in the proportion and absolute number of a classical dendritic cell subset, BDCA-1+ mDC-1 cells, especially in patients with high viral load at early stages of illness. This suggests that changes within the peripheral blood may be related to changes within the monocyte compartment. Monocytes are the predominant target for DENV infection in peripheral blood mononuclear cells, and monocyte activation has been implicated in DENV pathogenesis (Durbin et al., 2008Durbin A.P. Vargas M.J. Wanionek K. Hammond S.N. Gordon A. Rocha C. Balmaseda A. Harris E. Virology. 2008; 376: 429-435Crossref PubMed Scopus (156) Google Scholar). Expression of the surface markers CD14 and CD16 divides monocytes into three subsets that display distinct ligand sensing and cytokine/chemokine secretion patterns: "classical" CD14+CD16−, "intermediate" CD14+CD16+, and "nonclassical" CD14dimCD162+ monocytes (Wong et al., 2011Wong K.L. Tai J.J. Wong W.C. Han H. Sem X. Yeap W.H. Kourilsky P. Wong S.C. Blood. 2011; 118: e16-e31Crossref PubMed Scopus (649) Google Scholar). Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar found genes associated with the CD14+CD16+ monocyte subset to be enriched in patients with high viral load/early disease stage when monocyte-specific data sets were used to analyze the whole-blood transcriptome. These data were confirmed when monocyte populations were evaluated via flow cytometry in both humans and a nonhuman primate model of DENV infection. Nonhuman primates infected with DENV demonstrated an increase in CD14+CD16+ monocytes in peripheral blood between days 1 and 3 postinfection, and these cells were found in the lymph nodes by day 3. In addition, DENV infection of CD14+ monocytes from healthy human donors increased the frequency of CD14+CD16+ monocytes. These DENV-infected monocytes secreted MCP-1 (CCL-2), IP-10 (CXCL-10), interleukin-6 (IL-6), IL-8, and IL-10, similar to the pattern of cytokine and chemokine secretion observed in the plasma of patients with high viral load early in disease. These factors were previously shown to be associated with CD14+CD16+ monocytes (Wong et al., 2011Wong K.L. Tai J.J. Wong W.C. Han H. Sem X. Yeap W.H. Kourilsky P. Wong S.C. Blood. 2011; 118: e16-e31Crossref PubMed Scopus (649) Google Scholar). Taken together, these data provide strong evidence that DENV infection results in increased frequency of CD14+CD16+ monocytes and suggest that they may play a role in shaping the immune response to DENV. Further evaluation of DENV-infected nonhuman primates indicated that CD14+CD16+ monocytes express CD163 and CD169, markers of tissue subcapsular sinus macrophages. Subcapsular sinus macrophages localize to the subcapsular region of the lymph node and phagocytose viruses and stimulate virus-specific B cell responses (Junt et al., 2007Junt T. Moseman E.A. Iannacone M. Massberg S. Lang P.A. Boes M. Fink K. Henrickson S.E. Shayakhmetov D.M. Di Paolo N.C. et al.Nature. 2007; 450: 110-114Crossref PubMed Scopus (627) Google Scholar). To evaluate the ability of the CD14+CD16+ monocyte population to stimulate DENV-specific B cell responses, Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar cocultured DENV-infected CD14+CD16+ monocytes with autologous B cells. DENV-infected CD14+CD16+ monocytes stimulated resting B cells to differentiate into CD272+CD382+ plasmablasts that secreted immunoglobulin M (IgM) and IgG. Further analysis of the in vitro coculture system identified the B cell-modulating cytokines BAFF, APRIL, and IL-10, but not IL-6, as potential mechanisms by which this monocyte population stimulates plasmablast formation. Furthermore, expression of genes encoding BAFF and APRIL was increased in dengue patients with high viral load early in disease progression. These data suggest that early during DENV infection, monocytes differentiate into CD14+CD16+ monocytes and migrate to draining lymph nodes where they may encounter and activate antigen-specific memory B cells. In summary, Kwissa et al., 2014Kwissa M. Nakaya H.I. Onlamoon N. Wrammert J. Villinger F. Perng G.C. Yoksan S. Pattanapanyasat K. Chokephaibulkit K. Ahmed R. et al.Cell Host Microbe. 2014; 16 (this issue): 115-127Abstract Full Text Full Text PDF PubMed Scopus (157) Google Scholar utilized an elegant approach integrating immunoprofiling of DENV-infected human clinical samples with studies in nonhuman primates and in vitro coculture systems to identify a monocyte subset that modulates humoral responses (Figure 1). These novel data show that following DENV infection, CD14+CD16+ monocytes increase in the peripheral blood of both humans and nonhuman primates and then localize to the lymph nodes. Whether the increase in CD14+CD16+ monocytes is due to proliferation, mobilization from the bone marrow or spleen, or differentiation remains to be determined. The CD14+CD16+ monocytes express CD138 and CD169, surface markers of tissue subcapsular sinus macrophages, which in the lymph node capture viruses from tissues to present to B cells. The data suggest that this CD14+CD16+ monocyte population is capable of stimulating the expansion of CD382+CD272+ plasmablasts following DENV infection through the secretion of the cytokines BAFF, APRIL, and IL-10. These data provide an exciting new perspective into how the innate immune response plays a role in shaping the magnitude and quality of the adaptive immune response; future studies addressing the relation of these findings with disease severity are now warranted. The authors received no specific funding for this work. Related research in the laboratory of E.H. is supported by the US National Institutes of Health, the Bill and Melinda Gates Foundation, and the Instituto Carlos Slim de la Salud. Dengue Virus Infection Induces Expansion of a CD14+CD16+ Monocyte Population that Stimulates Plasmablast DifferentiationKwissa et al.Cell Host & MicrobeJune 26, 2014In BriefDengue infection in humans induces a potent inflammatory response and expansion of antibody-producing plasmablasts in the blood. By performing a systems biological analysis of innate responses to dengue, Kwissa et al. highlight a role for CD14+CD16+ monocytes in promoting the differentiation of plasmablasts and mediating antibody responses to dengue virus. Full-Text PDF Open Archive
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